RT Journal Article
T1 The 20-kDa chaperone-like protein of Bacillus thuringiensis ssp. israelensis enhances yield, crystal size and solubility of Cry3A
A1 Díaz Mendoza, María Mercedes
A1 Bideshi, Dennis
A1 Ortego, Felix
A1 Farinós, Gema
A1 Federici, Brian
AB Aims: To determine whether the 20-kDa chaperone-like protein of Bacillus thuringiensis ssp. israelensis enhances synthesis, crystallization and solubility of the Cry3A coleopteran toxin and whether the crystalline inclusions produced are toxic to neonates of the Colorado potato beetle, Leptinotarsa decemlineata.Methods and Results: The cry3A gene was expressed in the 4Q7 strain of B. thuringiensis ssp. israelensis in the absence or presence of the 20-kDa gene. The 20-kDa protein enhanced Cry3A yield by 2·7-fold per unit of fermentation medium. Crystal volumes averaged 2·123 and 0·964 μm3 when synthesized in, respectively, the presence or absence of the 20-kDa protein. Both crystals were soluble at pH 5 and pH 6; however, the larger crystal was 1·7× and 1·5× more soluble at, respectively, pH 7 and pH 10. No significant difference in toxicity against L. decemlineata neonates was observed.Conclusions: This report demonstrated that the 20-kDa chaperone-like protein enhances yield, volume and solubility of the coleopteran Cry3A crystalline inclusions per unit crystal/spore mixture.Significance and Impact of the Study: This is the first report showing that an accessory protein (20-kDa) could enhance synthesis and crystallization of Cry3A, a finding that could be beneficial for commercial production of this coleopteran-specific insecticidal protein for microbial insecticides and possibly even for transgenic crops.
PB Oxford University Press
SN 0266-8254
YR 2012
FD 2012
LK https://hdl.handle.net/20.500.14352/96190
UL https://hdl.handle.net/20.500.14352/96190
LA eng
NO Ministerio de Ciencia e Innovación (España)
NO National Institutes of Health
DS Docta Complutense
RD 8 abr 2025