RT Journal Article
T1 Development and validation of a liquid chromatography tandem massspectrometry method for the determination of cannabinoids andphase I and II metabolites in meconium
A1 Prego Meleiro, Pablo
A1 Lendoiro, Elena
A1 Concheiro, Marta
A1 Cruz, Angelines
A1 López-Rivadulla, Manuel
A1 De Castro, Ana
AB tA liquid chromatography–tandem mass spectrometry (LC–MSMS) method was developed and fullyvalidated for the determination of  9-tetrahydrocannabinol (THC), 11-hydroxyTHC (OHTHC), 11-nor-9-carboxyTHC (THCCOOH), 8- -11-dihydroxyTHC (diOHTHC), cannabinol, cannabidiol, and THC andTHCCOOH glucuronides in 0.25 ± 0.02 g meconium. Samples were homogenized in methanol and sub-jected to cation exchange solid-phase extraction. Chromatographic separation was performed on aKinetex C18 column (50 mm × 2.1 mm, 2.6  m) at 35◦C, with a gradient of 0.1% formic acid in waterand acetonitrile at a flow rate of 0.3 mL/min; total run time was 10 min. Two transitions per analyte weremonitored in MRM mode. The method was specific and sensitive; LOD was from 1 to 2 ng/g, and LOQfrom 4 to 10 ng/g; linearity ranged from 4 to 400 ng/g for all the analytes, except for THC glucuronide(10–400 ng/g); intra-assay, inter-assay and total imprecision were <11.2%, <13.45% and <15.6%, respec-tively; accuracy ranged from 93.9% to 109.0% of the target concentration; matrix effect, extraction andprocess efficiency ranged from −26.4% to −71.4%, 49.9% to 69.5% and 14.3% to 45.0%, respectively. Theinclusion of THC and THCCOOH glucuronides avoided the need for the hydrolysis process, thus facilitatingsample pretreatment. Application of the method to 19 authentic meconium specimens from uncontrolledpregnancies or women suspicious of drug consumption revealed fetal cannabis exposure in 4 newborns.THCCOOH (24.1–288.8 ng/g), diOHTHC (53.2–332.4 ng/g), THC (4.2–7.7 ng/g), CBN (30.7–93.3 ng/g) andCBD (7.1–251.5 ng/g) were detected in all cases; THCCOOH glucuronide (190.2–306.8 ng/g) in 3 cases; andOHTHC (11.9 ng/g) in the remaining one; however, THC glucuronide was not identified in any specimen.
PB Elsevier
YR 2017
FD 2017-03-25
LK https://hdl.handle.net/20.500.14352/116895
UL https://hdl.handle.net/20.500.14352/116895
LA eng
NO Prego-Meleiro P, Lendoiro E, Concheiro M, Cruz A, López-Rivadulla M, de Castro A. Development and validation of a liquid chromatography tandem mass spectrometry method for the determination of cannabinoids and phase I and II metabolites in meconium. J Chromatogr A. 2017 May 12;1497:118-126. doi: 10.1016/j.chroma.2017.03.066. Epub 2017 Mar 25. PMID: 28372838.
NO Ministerio de Sanidad (España)
NO Consellería de Cultura, Educación e Ordenación Universitaria, Xunta de Galicia,
DS Docta Complutense
RD 6 abr 2025