RT Journal Article
T1 Characterization of an atypical, thermostable, organic solvent- and acid-tolerant 2′-deoxyribosyltransferase from Chroococcidiopsis thermalis
A1 Del Arco, Jon
A1 Sánchez-Murcia, Pedro Alejandro
A1 Mancheño Gómez, José Miguel
A1 Gago, Federico
A1 Fernández Lucas, Jesús
AB In our search for thermophilic and acid-tolerant nucleoside 2′-deoxyribosyltransferases (NDTs), we found a good candidate in an enzyme encoded by Chroococcidiopsis thermalis PCC 7203 (CtNDT). Biophysical and biochemical characterization revealed CtNDT as a homotetramer endowed with good activity and stability at both high temperatures (50–100 °C) and a wide range of pH values (from 3 to 7). CtNDT recognizes purine bases and their corresponding 2′-deoxynucleosides but is also proficient using cytosine and 2′-deoxycytidine as substrates. These unusual features preclude the strict classification of CtNDT as either a type I or a type II NDT and further suggest that this simple subdivision may need to be updated in the future. Our findings also hint at a possible link between oligomeric state and NDT’s substrate specificity. Interestingly from a practical perspective, CtNDT displays high activity (80–100%) in the presence of several water-miscible co-solvents in a proportion of up to 20% and was successfully employed in the enzymatic production of several therapeutic nucleosides such as didanosine, vidarabine, and cytarabine.
PB Springer Nature
SN 0175-7598
YR 2018
FD 2018
LK https://hdl.handle.net/20.500.14352/109960
UL https://hdl.handle.net/20.500.14352/109960
LA eng
NO Del Arco, J., Sánchez-Murcia, P. A., Mancheño, J. M., Gago, F., & Fernández-Lucas, J. (2018). Characterization of an atypical, thermostable, organic solvent- and acid-tolerant 2′-deoxyribosyltransferase from Chroococcidiopsis thermalis. Applied Microbiology and Biotechnology, 102(16), 6947-6957. https://doi.org/10.1007/S00253-018-9134-Y
NO Ministerio de Economía y Competitividad (España)
NO Ministerio de Ciencia e Innovación (España)
NO Universidad Europea de Madrid
NO Fundación Santander
DS Docta Complutense
RD 10 abr 2025