RT Journal Article
T1 Expression and structural analysis of taste receptor genes in Iberian and Duroc pigs
A1 Óvilo, Cristina
A1 Benítez, Rita
A1 Núñez, Yolanda
A1 Peiró Pastor, Ramón
A1 García, Fabian
A1 De Mercado, Eduardo
A1 Gómez Izquierdo, Emilio
A1 García Casco, Juan
A1 López Bote, Clemente José
A1 Muñoz, María
AB Background: Taste receptor genes are expressed in sensory cells located in the tongue and influence food preferences, voluntary feed intake, and other relevant traits. Taste perception may differ between livestock breeds that show differences in eating behaviour and between animals that receive different diets or show phenotypic variation in feed intake or related-traits. The objectives of this work were to deepen the understanding of the regulation of the function of taste receptor genes in the circumvallate papillae of obese Iberian pigs in comparison to Duroc pigs, and to characterize their genetic variation and associations with relevant production traits.Results: We performed a gene expression and structural analysis of ten taste receptor genes in Iberian and Duroc pigs. Gene expression was quantified in the circumvallate papillae of 48 growing Iberian and Duroc pigs maintained under identical management conditions but fed isocaloric diets differing in energy source: either high concentration of fat rich in oleic acid (HO) or carbohydrates (CH); and sacrificed after 47 days of treatment (50.5 kg live weight). Gene expression differed between the two breeds for most of the analyzed genes, with the TAS1R1, TAS1R2, TAS1R3, TAS2R4, TAS2R38, TAS2R39, GPR84, and CD36 genes being overexpressed in Duroc pigs. The diet effect was modulated by breed, with TAS1R1, TAS1R3, and TAS2R4 genes being overexpressed only in Duroc pigs fed the HO diet. Detection of genetic variants (single nucleotide polymorphisms, SNPs) for this panel of genes was performed on muscle RNA-seq data, and three SNPs in the TAS1R1, TAS1R3, and CD36 genes were selected for association studies. All three SNPs were associated with various growth, fattening, tissue fat content, and composition traits. Moreover, the CD36:c.910G/T SNP was associated with oral CD36 gene expression and with differences in the predicted mRNA secondary structure.Conclusions: Most taste receptor genes are expressed at lower level in circumvallate papillae from Iberian than Duroc pigs. This aligns with lower overall taste sensitivity, higher feed intake, and obese nature of Iberian pigs. Significant association results were observed for SNPs in the TAS1R1 and TAS1R3 genes with meat quality traits and liver composition, which showed segregation in world-wide distributed breeds, but particularly for a potential causal SNP in the CD36 gene, associated with growth and tissue composition, which segregates in Iberian populations.
PB Springer
SN 0999-193X
YR 2025
FD 2025-05-02
LK https://hdl.handle.net/20.500.14352/120422
UL https://hdl.handle.net/20.500.14352/120422
LA eng
NO Óvilo, C., Benítez, R., Núñez, Y., Peiró-Pastor, R., García, F., De Mercado, E., Gómez-Izquierdo, E., García-Casco, J., López-Bote, C., & Muñoz, M. (2025). Expression and structural analysis of taste receptor genes in Iberian and Duroc pigs. Genetics, selection, evolution : GSE, 57(1), 22. https://doi.org/10.1186/s12711-025-00968-0
NO Contributions:CO and CLB designed the experiments and obtained funding. JGC, EGI and EM contributed to the design and development of the animal experiments. RB, YN and FG analyzed and interpreted the gene expression data. YN and FG performed the genotyping and MM analyzed the genotyping data and performed the statistical analysis of the associations. RPP performed the bioinformatics analysis of SNP discovery from RNA-seq data. CO wrote the manuscript. All the authors have read and approved the final manuscript.
NO Comunidad de Madrid
NO Ministerio de Ciencia, Innovación y Universidades (España)
DS Docta Complutense
RD 18 dic 2025