RT Journal Article
T1 Engineering of a live Salmonella enterica serovar Choleraesuis negative-marker strain that allows serological differentiation between immunised and infected animals
A1 Herrero-Gil, Aldara
A1 Carrión Herrero, Francisco Javier
A1 Orden Gutiérrez, José Antonio
A1 Fuente López, Ricardo De La
A1 Domínguez Bernal, Gustavo Ramón
AB The usefulness of Salmonella vaccine vehicles is limited by the fact that control programmes relying on Salmonella bacteriology and serology cannot differentiate infected animals from vaccinated ones, an ability referred to as DIVA (differentiating infected from vaccinated animals). As a first step towards Salmonella-based DIVA vaccines, the ompA gene was deleted in live attenuated ΔphoP and ΔrpoS vaccine strains. The ompA gene is present in all Salmonella enterica serovars and it encodes an abundant, highly immunogenic outer membrane protein. The double mutant ΔphoP ΔompA and ΔrpoS ΔompA strains showed similar virulence attenuation, safety and immunogenicity in a mouse model of infection as the parental ΔphoP and ΔrpoS strains. Sera from mice inoculated with the double mutant strains failed to recognise OmpA in Western blots of outer membrane extracts, whereas the protein was recognised by sera from mice inoculated with wild-type Salmonella or a mixture of double mutant and parental strains. These data suggest that OmpA can be a suitable negative marker for DIVA vaccines.
PB Elsevier
YR 2016
FD 2016
LK https://hdl.handle.net/20.500.14352/93742
UL https://hdl.handle.net/20.500.14352/93742
LA eng
NO Herrero-Gil, A., Carrión, J., Orden, J. A., de la Fuente, R., & Domínguez-Bernal, G. (2016). Engineering of a live Salmonella enterica serovar Choleraesuis negative-marker strain that allows serological differentiation between immunised and infected animals. Veterinary journal (London, England : 1997), 213, 53–58. https://doi.org/10.1016/j.tvjl.2016.03.016
NO Ministerio de Economía y Competitividad (España)
NO Ministerio de Educación y Ciencia e Innovación (España)
DS Docta Complutense
RD 6 abr 2025