RT Journal Article
T1 Multiscale porosity in mesoporous bioglass 3D-printed scaffolds for bone regeneration.
A1 Gómez Cerezo, María Natividad
A1 Peña López, Juan
A1 Ivanovski, Saso
A1 Arcos Navarrete, Daniel
A1 Vallet Regí, María Dulce Nombre
A1 Vaquette, Cedryck
AB In order to increase the bone forming ability of MBG-PCL composite scaffold, microporosity was created in the struts of 3D-printed MBG-PCL scaffolds for the manufacturing of a construct with a multiscale porosity consisting of meso- micro- and macropores. 3D-printing imparted macroporosity while the microporosity was created by porogen removal from the struts, and the MBG particles were responsible for the mesoporosity. The scaffolds were 3D-printed using a mixture of PCL, MBG and  phosphate buffered saline (PBS) particles, subsequently leached out.  Microporous-PCL (pPCL) as  a  negative control, microporous MBG-PCL (pMBG-PCL) and  non- microporous-MBG-PCL (MBG-PCL) were investigated. Scanning electron microscopy, mercury intrusion poros-imetry and micro-computed tomography demonstrated that the PBS removal resulted in the formation of mi-cropores inside the struts with porosity of around 30% for both pPCL and pMBG-PCL, with both constructs displaying an overall porosity of 8090%. In contrast, the MBG-PCL group had a microporosity of 6% and an overall porosity of 70%. Early mineralisation was found in the pMBG-PCL post-leaching out and this resulted in the formation a more homogeneous calcium phosphate layer when using a biomimetic mineralisation assay. Mechanical properties ranged from 5  to  25  MPa for  microporous and  non-microporous specimens, hence microporosity was the determining factor affecting compressive properties. MC3T3-E1 metabolic activity was increased in the pMBG-PCL along with an increased production of RUNX2. Therefore, the microporosity within a 3D-printed bioceramic composite construct may result in additional physical and biological benefits.
PB Elsevier
SN 0928-4931
YR 2020
FD 2020-11-04
LK https://hdl.handle.net/20.500.14352/7611
UL https://hdl.handle.net/20.500.14352/7611
LA eng
NO RESEARCHER ID L-6167-2014 (Daniel Arcos Navarrete)ORCID 0000-0002-5367-7272 (Daniel Arcos Navarrete)RESEARCHER ID M-3378-2014 (María Vallet Regí)ORCID 0000-0002-6104-4889 (María Vallet Regí)
NO Unión Europea. Horizonte 2020
NO Ministerio de Economía y Competitividad (MINECO)
DS Docta Complutense
RD 9 abr 2025