RT Journal Article
T1 A new hydrogel containing a proanthocyanidin polymer-rich extract of Commiphora leptophloeos for treating vulvovaginal candidiasis: Preclinical results using the alternative model of Galleria mellonella
A1 Dantas-Medeiros, Renato
A1 Davi Marena, Gabriel
A1 Sousa Araújo, Victor Hugo
A1 Bezerra Neto, Francisco de Assis
A1 Zanatta, Ana Caroline
A1 Peporine Lopes, Norberto
A1 Bermejo Benito, Paulina
A1 Guerra Guirao, José Antonio
A1 Bedoya Del Olmo, Luis Miguel
A1 Fonseca-Santos, Bruno
A1 Amorim-Carmo, Bruno
A1 Freitas Fernandes-Pedrosa, Matheus de
A1 Maranhão Chaves, Guilherme
A1 Bauab, Tais Maria
A1 Chorilli, Marlus
A1 Zucolotto, Silvana Maria
AB In the present study, we aimed to develop and characterize a hydrogel formulation containing a proanthocyanidin polymer-rich extract of Commiphora leptophloeos and investigate its toxicity and antifungal effect in vitro and in vivo on Galleria mellonella infected with Candida albicans larvae. The extract (4%) was incorporated into a hydrogel formulation based on chitosan (1.0%) and poloxamer 407 (18%), and evaluated by physical (organoleptic characteristics, rheological tests, and texture profile analysis), chemical (proanthocyanidin content and pH), and microbiological parameters. Under the study conditions, the new hydrogel displayed good physicochemical and microbiological stability for 3 months at 25 °C. Both the extract and the hydrogel were evaluated regarding their safety and efficacy in vitro on uterine/endometrial epithelial cells (HEC-1A) and against Candida spp. strains, as well as in vivo in the alternative model of acute toxicity and C. albicans-infection in G. mellonella. The results showed that the hydrogel is biocompatible with the vaginal environment, becoming more viscous with increasing temperature, and has higher antifungal potential compared to a free extract at all tested concentrations in vitro (2172–33.9 μg/mL), and in vivo at the dose of 125 mg/kg. Overall, the data provide evidence of the potential of the new hydrogel to be applied in treating vulvovaginal candidiasis.
PB Elsevier
SN 1773-2247
YR 2023
FD 2023-08
LK https://hdl.handle.net/20.500.14352/93129
UL https://hdl.handle.net/20.500.14352/93129
LA eng
NO Dantas-Medeiros R, Marena GD, Araújo VHS, Bezerra Neto FDA, Zanatta AC, Lopes NP, et al. A new hydrogel containing a proanthocyanidin polymer-rich extract of Commiphora leptophloeos for treating vulvovaginal candidiasis: Preclinical results using the alternative model of Galleria mellonella. Journal of Drug Delivery Science and Technology 2023;85:104531. https://doi.org/10.1016/j.jddst.2023.104531.
NO Conselho Nacional de Desenvolvimento Científico e Tecnologico
NO Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
NO Federal University of Rio Grande do Norte
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 16 abr 2025