TY  - JOUR
AU  - Siar, El Hocine
AU  - Abellanas Pérez, Pedro
AU  - Rocha Martín, Javier
AU  - Fernandez Lafuente, Roberto
PY  - 2024
DO  - 10.1016/j.ijbiomac.2024.134487
SN  - 0141-8130
UR  - https://hdl.handle.net/20.500.14352/112339
T2  - International Journal of Biological Macromolecules
AB  - Ficin has been immobilized at full loading on glyoxyl agarose beads. Then, ficin was blocked with 2,2′-dipyridyldisulfide. To be effective, the modification must be performed in the presence of 0.5 M urea, as the enzyme was not inhibited under...
LA  - eng
PB  - Elsevier
KW  - Substrate enzyme specificity tuning
KW  - Immobilization of proteases
KW  - Coimmobilizing inert proteins over proteases
TI  - Tailoring the specificity of ficin versus large hemoglobin and small casein by co-immobilizing inert proteins on the immobilized enzyme layer and further modification with aldehyde dextran
TY  - journal article
VL  - 277
ER  -