RT Journal Article
T1 Evaluation of techniques for post-mortem diagnosis of Mycobacterium tuberculosis complex infection in goats
A1 Jiménez Martín, Débora
A1 Muñoz Fernández, Leonor
A1 Agulló Ros, Irene
A1 Jiménez Pizarro, Natalia
A1 Cano Terriza, David
A1 García Bocanegra, Ignacio
A1 Romero Martínez, Beatriz
A1 Caballero Gómez, Javier
A1 Gonzálvez, Moisés
A1 Rejón, Salvador
A1 Martínez, Remigio
A1 Risalde, María A.
AB Goats are highly susceptible to Mycobacterium tuberculosis complex (MTBC) infection and can play an important role in the epidemiology of animal tuberculosis (TB) in certain epidemiological scenarios. Historically, culture has been considered the only gold standard technique for post-mortem confirmation of MTBC infection. However, it is constrained by its low sensitivity, the slow growth of MTBC, and stringent biosecurity requirements. Thus, alternative post-mortem techniques are essential for effective TB control and eradication. This study aimed to compare the use of different diagnosis techniques for post-mortem confirmation of MTBC infection in goats positive for official tuberculin skin tests (TST). The techniques evaluated were direct tissue real-time polymerase chain reaction (RT-PCR), culture, observation of gross and histopathological tuberculosis-like lesions (TBL), and Ziehl-Neelsen staining. Lung and retropharyngeal and mediastinal lymph node (LN) samples were taken from 205 TST-positive goats. Direct tissue RT-PCR showed the highest rate of positive animals for post-mortem confirmation of TB infection (86.3 %) after the official in vivo diagnostic test, followed by histopathological (49.3 %), and gross (47.8 %) observation of TBL, MTBC culture (42.4 %) and ZN staining (8.5 %). Spoligotyping was obtained for 86 of the mycobacteria cultures, revealing SB0157 in 86.0 % (74/86) and SB1081 in 15.1 % (13/86) of them, both belonging to Mycobacterium caprae. Histopathological study showed a predominance of necrotic TBL in 85.2 % (52/61) and 83.5 % (66/79) of the granulomas observed in lung and LN samples, respectively. Our results indicate that direct RT-PCR on tissue is a sensitive and reliable technique for post-mortem confirmation of MTBC infection in the caprine TB control and eradication programs, even in animals without TBL
PB Elsevier
SN 0378-1135
YR 2025
FD 2025-05
LK https://hdl.handle.net/20.500.14352/119171
UL https://hdl.handle.net/20.500.14352/119171
LA eng
NO Jiménez-Martín, D., Muñoz-Fernández, L., Agulló-Ros, I., Jiménez-Pizarro, N., Cano-Terriza, D., García-Bocanegra, I., Romero, B., Caballero-Gómez, J., Gonzálvez, M., Rejón, S., Martínez, R., & Risalde, M. A. (2025). Evaluation of techniques for post-mortem diagnosis of Mycobacterium tuberculosis complex infection in goats. Veterinary microbiology, 304, 110485. Advance online publication. https://doi.org/10.1016/j.vetmic.2025.110485
NO Credit authorship contribution statementCaballero-Gómez Javier: Writing – review & editing, Writing – original draft, Visualization, Validation, Software, Formal analysis, Data curation. Gonzálvez Moisés: Writing – review & editing, Writing – original draft, Software, Resources, Investigation, Formal analysis, Data curation. Rejón Salvador: Writing – review & editing, Writing – original draft, Visualization, Validation, Resources, Investigation. Martínez Remigio: Writing – review & editing, Writing – original draft, Visualization, Validation, Supervision, Software, Resources, Project administration, Methodology, Investigation, Funding acquisition, Formal analysis, Data curation, Conceptualization. Risalde María A.: Writing – review & editing, Writing – original draft, Visualization, Validation, Supervision, Software, Resources, Project administration, Methodology, Investigation, Funding acquisition, Formal analysis, Data curation, Conceptualization. Jiménez-Martín Débora: Writing – review & editing, Writing – original draft, Visualization, Validation, Methodology, Investigation, Formal analysis, Data curation, Conceptualization. Muñoz-Fernández Leonor: Writing – review & editing, Writing – original draft, Visualization, Software, Investigation, Formal analysis, Data curation. Agulló-Ros Irene: Writing – review & editing, Writing – original draft, Visualization, Validation, Software, Resources, Methodology, Formal analysis, Data curation. Jiménez-Pizarro Natalia: Writing – review & editing, Writing – original draft, Visualization, Validation, Methodology, Investigation, Formal analysis, Data curation. Cano-Terriza David: Writing – review & editing, Writing – original draft, Visualization, Validation, Software, Methodology, Investigation, Formal analysis, Data curation, Conceptualization. García-Bocanegra Ignacio: Writing – review & editing, Writing – original draft, Visualization, Validation, Supervision, Resources, Project administration, Investigation, Funding acquisition, Formal analysis, Data curation. Romero Beatriz: Writing – review & editing, Writing – original draft, Visualization, Validation, Methodology, Formal analysis, Data curation.
NO Universidad de Córdoba (España)
NO CIBER (Centro de Investigación Biomédica en Red)
NO Ministerio de Universidades (España)
NO European Commission
DS Docta Complutense
RD 11 abr 2025