RT Journal Article
T1 All-in-one silk-fibroin sustainable high performance electrochemical immunoplatforms: determination of the protein TIM-1 in cancer and allergy scenarios as a case study
A1 Santiago, Sara
A1 Cabrero Martín, Andrea
A1 Serafín González-Carrato, Verónica
A1 Pedrero Muñoz, María
A1 Munõz Berbel, X.
A1 Guirado, G.
A1 Montero Calle, Ana
A1 Pingarrón Carrazón, José Manuel
A1 Barderas Manchado, Rodrigo
A1 Campuzano Ruiz, Susana
AB Silk fibroin (SF) is a natural biopolymer extracted from Bombyx mori that has emerged as a promising sustainable material in biosensing applications, especially because of its biodegradable and tunable properties. However, SF is still insufficiently explored in electrochemical biosensing. We present a novel all-in-one electrochemical immunoplatform basedon a disposable electrode surface sequentially modified with Prussian Blue (PB) and SF. First, PB is electrodeposited, acting as a redox mediator that enhances signal amplification through its reversible electrochemistry and its low reduction potential for H2O2. The SF layer is here presented as a novel, biocompatible matrix for antibody immobilization thatoffers structural versatility and sustainability. This dual modification generates a synergistic ecofriendly functional interface that is stable at room temperature. Onto this innovative surface, we have developed a sandwich-type electrochemical immunosensor employing a biotinylated detector antibody and streptavidin–horseradish peroxidase (Strep-HRP) as theenzymatic label for the determination of T-cell immunoglobulin and mucin-domain containing protein 1 (TIM-1, also known as KIM-1 or HAVCR1), a relevant biomarker associated with cancer-related angiogenesis and poor prognosis, and, more recently, with allergenic episodes. The platform enabled sensitive and selective determination of TIM-1 in plasmasamples from colorectal cancer (CRC) patients, and saliva samples from individuals undergoing allergic episodes, in which elevated TIM-1 levels were observed. A remarkable advantage of this immunosensor is its operational stability for up to 20 days at room temperature, eliminating the need for a cold chain.
PB Springer
YR 2026
FD 2026-04-20
LK https://hdl.handle.net/20.500.14352/139028
UL https://hdl.handle.net/20.500.14352/139028
LA eng
NO S. Santiago*, A. Cabrero-Martín, V. Serafín, M. Pedrero, X. Munõz-Berbel, G. Guirado, A. Montero-Calle, J.M. Pingarrón, R. Barderas, S. Campuzano*. “All-in-one silk-fibroin sustainable high performance electrochemical immunoplatforms: determination of the protein TIM-1 in cancer and allergy scenarios as a case study”. Microchimica Acta 193 (2026) 322. https://doi.org/10.1007/s00604-026-08049-9
NO Complutense University of Madrid
NO Ministerio de Ciencia e Innovación
NO Generalitat de Catalunya
DS Docta Complutense
RD 20 ago 2026