%0 Journal Article
%A Lacadena García-Gallo, Francisco Javier
%A Martínez Del Pozo, Álvaro
%A Valle Lacadena
%A Martínez Ruiz, Antonio
%A Mancheño Gómez, José Miguel
%A Oñaderra Sánchez, Mercedes
%A Gavilanes Franco, José Gregorio
%T The cytotoxin α‐sarcin behaves as a cyclizing ribonuclease
%D 1998
%@ 0014-5793
%@ 1873-3468
%U https://hdl.handle.net/20.500.14352/103372
%X The hydrolysis of adenylyl(3PC5P)adenosine (ApA)and guanylyl(3PC5P)adenosine (GpA) dinucleotides by thecytotoxic protein K-sarcin has been studied. Quantitativeanalysis of the reaction has been performed through reversephase chromatographic (HPLC) separation of the resultingproducts. The hydrolysis of the 3P-5P phosphodiester bond ofthese substrates yields the 2P-3P cyclic mononucleotide; thisintermediate is converted into the corresponding 3P-monophosphate derivative as the final product of the reaction. Thevalues of the apparent Michaelis constant (KM), kcat and kcat/KM have also been calculated. The obtained results fit into a twostep mechanism for the enzymatic activity of K-sarcin and allowto consider this protein as a cyclizing RNase.z 1998 Federation of European Biochemical Societies.
%~