RT Journal Article
T1 The cytotoxin α‐sarcin behaves as a cyclizing ribonuclease
A1 Lacadena García-Gallo, Francisco Javier
A1 Martínez Del Pozo, Álvaro
A1 Valle Lacadena, 
A1 Martínez Ruiz, Antonio
A1 Mancheño Gómez, José Miguel
A1 Oñaderra Sánchez, Mercedes
A1 Gavilanes Franco, José Gregorio
AB The hydrolysis of adenylyl(3PC5P)adenosine (ApA)and guanylyl(3PC5P)adenosine (GpA) dinucleotides by thecytotoxic protein K-sarcin has been studied. Quantitativeanalysis of the reaction has been performed through reversephase chromatographic (HPLC) separation of the resultingproducts. The hydrolysis of the 3P-5P phosphodiester bond ofthese substrates yields the 2P-3P cyclic mononucleotide; thisintermediate is converted into the corresponding 3P-monophosphate derivative as the final product of the reaction. Thevalues of the apparent Michaelis constant (KM), kcat and kcat/KM have also been calculated. The obtained results fit into a twostep mechanism for the enzymatic activity of K-sarcin and allowto consider this protein as a cyclizing RNase.z 1998 Federation of European Biochemical Societies.
SN 0014-5793
SN 1873-3468
YR 1998
FD 1998-03-06
LK https://hdl.handle.net/20.500.14352/103372
UL https://hdl.handle.net/20.500.14352/103372
LA eng
NO Lacadena Javier, Martı́nez del Pozo Alvaro, Lacadena Valle, Martı́nez-Ruiz Antonio, Mancheño José M, Oñaderra Mercedes and Gavilanes José G(1998), The cytotoxin α-sarcin behaves as a cyclizing ribonuclease, FEBS Letters, 424, doi: 10.1016/S0014-5793(98)00137-9
DS Docta Complutense
RD 7 oct 2024