RT Journal Article
T1 Macroglial and retinal changes in hypercholesterolemic rabbits after normalization of cholesterol levels
A1 Ramírez Sebastián, Ana Isabel
A1 Salazar Corral, Juan José
A1 Hoz Montañana, María Rosa De
A1 Rojas López, María Blanca
A1 Ruiz Olivar, José Emilio
A1 Tejerina Sánchez, María Teresa
A1 Ramírez Sebastián, José Manuel
A1 Triviño Casado, Alberto
AB This study evaluates hypercholesterolemic rabbits, examining the retinal changes in Müller cells and astrocytes as well as their variations after a period of normal blood-cholesterol values induced by a standard diet. New Zealand rabbits were divided into three groups: G0, fed a standard diet; G1A, fed a 0.5% cholesterol-enriched diet for 8 months; and G1B, fed as G1A followed by standard diet for 6 months. Eyes were processed for transmission electron microscopy and immunohistochemistry (GFAP). While G1B resembled G0 more than did G1A, they shared alterations with G1A: a) as in G1A, Müller cells were GFAP+, filled spaces left by axonal degeneration, formed glial scars and their nuclei were displaced to the nerve-fibre layer. The area occupied by the astrocytes associated with the nerve-fibre bundles (AANFB) and by perivascular astrocytes (PVA) in G1A and G1B was significantly lower than in controls. However, no significant differences in PVA were found between G1A and G1B. In G1B, type I PVA was absent and replaced by hypertrophic type II cells; b) Bruch's membrane (BM) was thinner in G1B than in G1A; c) the retinal pigment epithelium (RPE) cytoplasm contained fewer lipids in G1B than in G1A; d) in G1A and G1B choriocapillaris and retinal vessel showed alterations with respect to G0; e) cell death and axonal degeneration in the retina were similar in G1A and G1B. The substitution of a hyperlipemic diet by a standard one normalizes blood-lipid levels. However, the persistence of damage at retinal vessels and BM-RPE could trigger chronic ischemia.
PB Elsevier
SN 0014-4835
YR 2006
FD 2006-09-26
LK https://hdl.handle.net/20.500.14352/51982
UL https://hdl.handle.net/20.500.14352/51982
LA eng
NO Ramírez Sebastián, A. I., Salazar Corral, J. J., Hoz Montañana, M. R. et al. «Macroglial and Retinal Changes in Hypercholesterolemic Rabbits after Normalization of Cholesterol Levels». Experimental Eye Research, vol. 83, n.o 6, diciembre de 2006, pp. 1423-38. DOI.org (Crossref), https://doi.org/10.1016/j.exer.2006.07.023.
NO Received 4 May 2006; accepted in revised form 22 July 2006Available online 26 September 2006
NO Comunidad de Madrid
NO Universidad Complutense de Madrid
NO Instituto Salud Carlos III
DS Docta Complutense
RD 10 abr 2025