RT Journal Article
T1 In vitro biofilm formation on different ceramic biomaterial surfaces: Coating with two bactericidal glasses
A1 Llama Palacios, María Arantxazu
A1 Sánchez Beltrán, María Del Carmen
A1 Díaz, Luis Antonio
A1 Cabal, Belén
A1 Suárez, Marta
A1 Moya, José S.
A1 Torrecillas, Ramón
A1 Figuero Ruiz, Elena
A1 Sanz Alonso, Mariano
A1 Herrera González, David
AB Objectives: To compare biofilm formation on the surface of different ceramic biomaterials to be used in implant dentistry.Methods: In vitro biofilm formation was investigated from mixtures of standard reference strains of Streptococcus oralis, Veillonella parvula, Actinomyces naeslundii, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis. Sterile ceramic calcium hydroxyapatite discs (HA) as control, sterile Al2O3/Ce-TZP nanocomposite sandblasted discs (material A1) and sterile Al2O3/Ce-TZP nanocomposite sandblasted discs and coated with two types of antimicrobial glasses (materials A2 and A3) were used. Biofilms were grown on the four surfaces and evaluated after 12, 24, 48 and 72 h of incubation. Biofilms were examined by confocal laser scanning microscopy (CLSM). In addition, counts of live bacterial cells of the target species A. actinomycetemcomitans, F. nucleatum and P. gingivalis were calculated by quantitative polymerase chain reaction (qPCR) combined with propidium monoazide (PMA). For data analysis, bacterial counts were compared with a multivariate general lineal model.Results: Using CLSM, cell vitality decreased in A2 and A3. With qPCR-PMA, significant differences in vitality were observed forA. actinomycetemcomitans in A3 after 48 and 72 h of incubation. With respect to the development of the biofilms, a significant increase in counts on HA and materials A1 and A2 was observed for A. actinomycetemcomitans and F. nucleatum. Conversely, for P. gingivalis, no differences were found for HA and materials A1 and A2.Significance: Differences in biofilm formation were detected among the different tested materials. The ceramic material A3 has an effect on the vitality of A. actinomycetemcomitans growing in an in vitro biofilm model.
PB Elsevier
SN 0109-5641
YR 2019
FD 2019-06
LK https://hdl.handle.net/20.500.14352/109006
UL https://hdl.handle.net/20.500.14352/109006
LA eng
NO Llama-Palacios A, Sánchez MC, Díaz LA, Cabal B, Suárez M, Moya JS, Torrecillas R, Figuero E, Sanz M, Herrera D. In vitro biofilm formation on different ceramic biomaterial surfaces: Coating with two bactericidal glasses. Dent Mater. 2019 Jun;35(6):883-892. doi: 10.1016/j.dental.2019.03.003
NO Ministerio de Economía, Comercio y Empresa (España)
NO Cátedra extraordinaria Dentaid-UCM
DS Docta Complutense
RD 28 abr 2025