RT Journal Article
T1 Dual detection system for cancer-associated point mutations assisted by a multiplexed LNA-based amperometric bioplatform coupled with rolling circle amplification
A1 Sebuyoya, Ravery
A1 Valverde De La Fuente, Alejandro
A1 Moranova, Ludmila
A1 Strmiskova, Johana
A1 Hrstka, Roman
A1 Ruiz Valdepeñas Montiel, Víctor
A1 Pingarrón Carrazón, José Manuel
A1 Barderas Manchado, Rodrigo
A1 Campuzano Ruiz, Susana
A1 Martin Bartosik, 
AB DNA point mutation in a BRAF proto-oncogene, V600E, is considered an important prognostic and predictive biomarker in various types of cancer, such as melanoma or colorectal cancer. We report here a novel electrochemical (EC) bioplatform for the analysis of BRAF V600E mutation coupled with rolling circle amplification (RCA) and locked nucleic acid (LNA) capture probes. A dual detection system was implemented, whereby two padlock probes complementary to either wild-type (wt) BRAF gene or DNA with V600E mutation (mut) led to amplification of wt or mut variant, respectively. Hybridization with specific LNA capture probes then increased the assay specificity, while EC detection provided rapid measurement times. The bioplatform was applied to analyze BRAF V600E mutation of cancer cells and tumor tissues from patients with melanoma or colorectal cancer. This is the first RCA-based EC bioplatform for BRAF analysis in a dual format without using PCR or sophisticated instrumentation.
PB Elsevier
YR 2023
FD 2023-07-27
LK https://hdl.handle.net/20.500.14352/123879
UL https://hdl.handle.net/20.500.14352/123879
LA eng
NO Ravery Sebuyoya, Alejandro Valverde, Ludmila Moranova, Johana Strmiskova, Roman Hrstka, Víctor Ruiz-Valdepeñas Montiel, José M. Pingarrón, Rodrigo Barderas, Susana Campuzano, Martin Bartosik, Dual detection system for cancer-associated point mutations assisted by a multiplexed LNA-based amperometric bioplatform coupled with rolling circle amplification, Sensors and Actuators B: Chemical, Volume 394, 2023, 134375, ISSN 0925-4005, https://doi.org/10.1016/j.snb.2023.134375.
NO Czech Health Research Council
NO Spanish Ministerio de Ciencia e Innovacion
NO TRANSNANOAVANSENS-CM
NO AES-ISCIII Program co-founded by FEDER funds
DS Docta Complutense
RD 22 mar 2026