RT Journal Article T1 Recent advances in practical methods for liver cell biology: a short overview A1 Torres, Sandra A1 Abdullah, Zeinab A1 Brol, Maximilian J. A1 Hellerbrand, Claus A1 Fernández, Mercedes A1 Fiorotto, Romina A1 Klein, Sabine A1 Königshofer, Philipp A1 Liedtke, Christian A1 Lotersztajn, Sophie A1 Nevzorova, Yulia A1 Schierwagen, Robert A1 Reiberger, Thomas A1 Uschner, Frank Erhard A1 Tacke, Frank A1 Weiskirchen, Ralf A1 Trebicka, Jonel AB Molecular and cellular research modalities for the study of liver pathologies have been tremendously improved over the recent decades. Advanced technologies offer novel opportunities to establish cell isolation techniques with excellent purity, paving the path for 2D and 3D microscopy and high-throughput assays (e.g., bulk or single-cell RNA sequencing). The use of stem cell and organoid research will help to decipher the pathophysiology of liver diseases and the interaction between various parenchymal and non-parenchymal liver cells. Furthermore, sophisticated animal models of liver disease allow for the in vivo assessment of fibrogenesis, portal hypertension and hepatocellular carcinoma (HCC) and for the preclinical testing of therapeutic strategies. The purpose of this review is to portray in detail novel in vitro and in vivo methods for the study of liver cell biology that had been presented at the workshop of the 8th meeting of the European Club for Liver Cell Biology (ECLCB-8) in October of 2018 in Bonn, Germany. PB MDPI SN 1661-6596, ESSN: 1422-0067 YR 2020 FD 2020-03-16 LK https://hdl.handle.net/20.500.14352/6286 UL https://hdl.handle.net/20.500.14352/6286 LA eng NO Unión Europea. Horizonte 2020 NO Deutsche Forschungsgemeinschaft NO European Association for the Study of the Liver (ESAL) NO European Foundation for the study of chronic liver failure (EFCLIF) NO Bayer DS Docta Complutense RD 5 abr 2025