RT Journal Article
T1 In vivo quantification of volatile organoselenium compounds released by bacteria exposed to selenium with HS-SPME-GC-MS. Effect of selenite and selenium nanoparticles
A1 Moreno Martín, Gustavo
A1 Sanz Landaluce, Jon
A1 León González, María Eugenia De
A1 Madrid Albarrán, María Yolanda
A2 Elsevier, 
AB Quantification of volatile organoselenium species released by Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), after their growth in the presence of 1 and 2 mg Se⋅L-1 as both selenite and chitosan modified selenium nanoparticles (Ch-SeNPs), was achieved by the application of a method based on headspace solid-phase microextraction (HS-SPME) and in-fiber internal standardization, combined with gas chromatography coupled to mass spectrometry (GC-MS). This method consisted of an initial extraction of the released volatile organoselenium compounds on the SPME fiber, followed by the extraction of internal standard (IS), deuterated dimethyl sulfide (d6-DMS), on the same fiber before its desorption at the injection port of GC-MS. The results showed that the biotransformation of selenite and Ch-SeNPs into volatile organoselenium compounds was dependent on both the type of bacterial species and the chemical form of selenium (Se) administered. In this sense, E. coli was able to biotransform both selenite and Ch-SeNPs into dimethylselenium (DMSe) and dimethyldiselenium (DMDSe) while S. aureus, biotransformed selenite into DMSe and DMDSe and, Ch-SeNPs only into DMDSe. Additionally, the formation of a volatile mixed sulfur/selenium compound, dimethyl selenenyl sulfide (DMSeS), from Se in nanoparticulated form has been detected for the first time.
PB Elsevier
SN 0039-9140
YR 2021
FD 2021-03-01
LK https://hdl.handle.net/20.500.14352/92504
UL https://hdl.handle.net/20.500.14352/92504
LA eng
NO Moreno-Martín, G; Sanz-Landaluze, J; León-González, ME; Madrid, Y. In vivo quantification of volatile organoselenium compounds released by bacteria exposed to selenium with HS-SPME-GC-MS. Effect of selenite and selenium nanoparticles 2021, 224: 121907
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Fondo Europeo de Desarrollo Regional
NO Universidad Complutense de Madrid
NO Comunidad de Madrid
NO Ministerio de Salud (España) - Formación Sanitaria Especializada
DS Docta Complutense
RD 3 jul 2024