RT Journal Article
T1 Epithelial CD47 is critical for mucosal repair in the murine intestine in vivo
A1 Azcutia Criado, Verónica
A1 Reed, Michelle
A1 Luissint, Anny-Claude
A1 Fan, Shuling
A1 O'Leary, Monique N.
A1 Quiros, Miguel
A1 Brazil, Jennifer
A1 Nusrat, Asma
A1 Parkos, Charles A.
AB CD47 is a ubiquitously expressed transmembrane glycoprotein that regulates inflammatory responses and tissue repair. Here, we show that normal mice treated with anti-CD47 anti- bodies, and Cd47-null mice have impaired intestinal mucosal wound healing. Furthermore, intestinal epithelial cell (IEC)-specific loss of CD47 does not induce spontaneous immune- mediated intestinal barrier disruption but results in defective mucosal repair after biopsy- induced colonic wounding or Dextran Sulfate Sodium (DSS)-induced mucosal damage. In vitro analyses using primary cultures of CD47-deficient murine colonic IEC or human colonoid-derived IEC treated with CD47-blocking antibodies demonstrate impaired epithelial cell migration in wound healing assays. Defective wound repair after CD47 loss is linked to decreased epithelial β1 integrin and focal adhesion signaling, as well as reduced thrombospondin-1 and TGF-β1. These results demonstrate a critical role for IEC-expressed CD47 in regulating mucosal repair and raise important considerations for possible alterations in wound healing secondary to therapeutic targeting of CD47.
PB Nature Portfolio
YR 2019
FD 2019
LK https://hdl.handle.net/20.500.14352/117141
UL https://hdl.handle.net/20.500.14352/117141
LA eng
NO Reed, Michelle, et al. «Epithelial CD47 Is Critical for Mucosal Repair in the Murine Intestine in Vivo». Nature Communications, vol. 10, n.o 1, noviembre de 2019, p. 5004. DOI.org (Crossref), https://doi.org/10.1038/s41467-019-12968-y
NO National Institute of Health (US)
NO Crohns and Colitis Foundation (US)
DS Docta Complutense
RD 7 jun 2025