RT Journal Article
T1 Two-step enzymatic strategy for the synthesis of a smart phenolic polymer and further immobilization of a β-galactosidase able to catalyze transglycosydation reaction
A1 Levin, Gustavo
A1 Gómez, Sara
A1 Glodowsky, Alejandro
A1 Cascone, Osvaldo
A1 Hernáiz Gómez-Degano, María Josefa
AB A rapid and efficient enzymatic procedure for the preparation of an immobilized beta-galactosidase has been described. In a first step, soybean peroxidase was used to catalyze the polymerization of a strategically activated phenol (N-Succinimidyl 3-(4-hydroxyphenyl)propionate, known as Bolton-Hunter reagent). The phenolic support was directly employed for immobilizing S-galactosidase from Bacillus circulans (ATCC 31382, beta-Gal-3), giving rise to a new biocatalyst subsequently applied in the synthesis of a beta-galatodisaccharide (Gal-beta(1-3)-GlcNAc and Gal-beta(1-3)-GalNAc). The reaction proceeded with high conversion rates and total regioselectivity. Reusability assays were performed with the same reaction conditions finding that the immobilized enzyme retains about 55% of its activity after eight batches. Finally and based on our results, the two-step enzymatic procedure presented here is a good and green alternative to the preparation of carbohydrates with biological activities.
PB Elsevier
SN 0141-8130
YR 2018
FD 2018
LK https://hdl.handle.net/20.500.14352/95781
UL https://hdl.handle.net/20.500.14352/95781
LA eng
NO Levin, G., et al. «Two-Step Enzymatic Strategy for the Synthesis of a Smart Phenolic Polymer and Further Immobilization of a β-Galactosidase Able to Catalyze Transglycosydation Reaction». International Journal of Biological Macromolecules, vol. 117, octubre de 2018, pp. 264-70. https://doi.org/10.1016/j.ijbiomac.2018.05.177.
NO Ministerio de Economía, Comercio y Empresa (España)
DS Docta Complutense
RD 10 nov 2025