RT Journal Article
T1 Efficient Dissection and Culture of Primary Mouse Retinal Pigment Epithelial Cells
A1 Chinchilla Rodríguez, Blanca
A1 Getachew, Heran
A1 Fernandez Godino, Rosario
AB Eye disorders affect millions of people worldwide, but the limited availability of human tissues hinders their study. Mouse models are powerful tools to understand the pathophysiology of ocular diseases because of their similarities with human anatomy and physiology. Alterations in the retinal pigment epithelium (RPE), including changes in morphology and function, are common features shared by many ocular disorders. However, successful isolation and culture of primary mouse RPE cells is very challenging. This paper is an updated audiovisual version of the protocol previously published by Fernandez-Godino et al. in 2016 to efficiently isolate and culture primary mouse RPE cells. This method is highly reproducible and results in robust cultures of highly polarized and pigmented RPE monolayers that can be maintained for several weeks on Transwells. This model opens new avenues for the study of the molecular and cellular mechanisms underlying eye diseases. Moreover, it provides a platform to test therapeutic approaches that can be used to treat important eye diseases with unmet medical needs, including inherited retinal disorders and macular degenerations.
PB MyJove Corporation
SN 1940-087X
YR 2021
FD 2021-02-10
LK https://hdl.handle.net/20.500.14352/116675
UL https://hdl.handle.net/20.500.14352/116675
LA eng
NO Chinchilla, B., Getachew, H., & Fernandez-Godino, R. (2021). Efficient dissection and culture of primary mouse retinal pigment epithelial cells. Journal of Visualized Experiments, 2021(168), 1-10. https://doi.org/10.3791/62228
DS Docta Complutense
RD 8 abr 2025