RT Journal Article
T1 HvPap-1 C1A protease actively participates in barley proteolysis mediated by abiotic stresses
A1 Velasco-Arroyo, Blanca
A1 Díaz Mendoza, María Mercedes
A1 Gandullo, Jacinto
A1 Gonzalez-Melendi, Pablo
A1 Santamaria, Estrella
A1 Dominguez-Figueroa, Jose
A1 Hensel, Goetz
A1 Martinez, Manuel
A1 Kumlehn, Jochen
A1 Diaz, Isabel
AB Protein breakdown and mobilization from old or stressed tissues to growing and sink organs are some of the metabolic features associated with abiotic/biotic stresses, essential for nutrient recycling. The massive degradation of proteins implies numerous proteolytic events in which cysteine-proteases are the most abundant key players. Analysing the role of barley C1A proteases in response to abiotic stresses is crucial due to their impact on plant growth and grain yield and quality. In this study, dark and nitrogen starvation treatments were selected to induce stress in barley. Results show that C1A proteases participate in the proteolytic processes triggered in leaves by both abiotic treatments, which strongly induce the expression of the HvPap-1 gene encoding a cathepsin F-like protease. Differences in biochemical parameters and C1A gene expression were found when comparing transgenic barley plants overexpressing or silencing the HvPap-1 gene and wild-type dark-treated leaves. These findings associated with morphological changes evidence a lifespan-delayed phenotype of HvPap-1 silenced lines. All these data elucidate on the role of this protease family in response to abiotic stresses and the potential of their biotechnological manipulation to control the timing of plant growth.
PB Oxford University Press
SN 0022-0957
YR 2016
FD 2016
LK https://hdl.handle.net/20.500.14352/95766
UL https://hdl.handle.net/20.500.14352/95766
LA eng
NO Blanca Velasco-Arroyo, Mercedes Diaz-Mendoza, Jacinto Gandullo, Pablo Gonzalez-Melendi, M. Estrella Santamaria, Jose D. Dominguez-Figueroa, Goetz Hensel, Manuel Martinez, Jochen Kumlehn, Isabel Diaz, HvPap-1 C1A protease actively participates in barley proteolysis mediated by abiotic stresses, Journal of Experimental Botany, Volume 67, Issue 14, July 2016, Pages 4297–4310, https://doi.org/10.1093/jxb/erw212
NO Ministerio de Economia y Competitividad (España)
NO European Commission
DS Docta Complutense
RD 30 dic 2025