%0 Journal Article
%A Li, Shaoguang
%A Ferrer-Ruiz, Andrés
%A Dai, Jun
%A Ramos-Soriano, Javier
%A Du, Xuewei
%A Zhu, Man
%A Zhang, Wanxue
%A Wang, Yuanyuan
%A Herranz Astudillo, María Ángeles
%A Jing, Le
%A Zhang, Zishuo
%A Li, Hui
%A Xia, Fan
%A Martín León, Nazario
%T A pH-independent electrochemical aptamer-based biosensor supports quantitative, real-time measurement in vivo
%D 2022
%U https://hdl.handle.net/20.500.14352/120027
%X The development of biosensors capable of achieving accurate and precise molecular measurements in the living body in pH-variable biological environments (e.g. subcellular organelles, biological fluids and organs) plays a significant role in personalized medicine. Because they recapitulate the conformation-linked signaling mechanisms, electrochemical aptamer-based (E-AB) sensors are good candidates to fill this role. However, this class of sensors suffers from a lack of a stable and pH-independent redox reporter to support their utility under pH-variable conditions. Here, in response, we demonstrate the efficiency of an electron donor p-extended tetrathiafulvalene (exTTF) as an excellent candidate (due to its good electrochemical stability and no proton participation in its redox reaction) of pH-independent redox reporters. Its use has allowed improvement of E-AB sensing performance in biological fluids under different pH conditions, achieving high-frequency, real-time molecular measurements in biological samples both in vitro and in the bladders of living rats.
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