RT Journal Article
T1 A pH-independent electrochemical aptamer-based biosensor supports quantitative, real-time measurement in vivo
A1 Li, Shaoguang
A1 Ferrer-Ruiz, Andrés
A1 Dai, Jun
A1 Ramos-Soriano, Javier
A1 Du, Xuewei
A1 Zhu, Man
A1 Zhang, Wanxue
A1 Wang, Yuanyuan
A1 Herranz Astudillo, María Ángeles
A1 Jing, Le
A1 Zhang, Zishuo
A1 Li, Hui
A1 Xia, Fan
A1 Martín León, Nazario
AB The development of biosensors capable of achieving accurate and precise molecular measurements in the living body in pH-variable biological environments (e.g. subcellular organelles, biological fluids and organs) plays a significant role in personalized medicine. Because they recapitulate the conformation-linked signaling mechanisms, electrochemical aptamer-based (E-AB) sensors are good candidates to fill this role. However, this class of sensors suffers from a lack of a stable and pH-independent redox reporter to support their utility under pH-variable conditions. Here, in response, we demonstrate the efficiency of an electron donor p-extended tetrathiafulvalene (exTTF) as an excellent candidate (due to its good electrochemical stability and no proton participation in its redox reaction) of pH-independent redox reporters. Its use has allowed improvement of E-AB sensing performance in biological fluids under different pH conditions, achieving high-frequency, real-time molecular measurements in biological samples both in vitro and in the bladders of living rats.
PB Royal Society of Chemistry
YR 2022
FD 2022-06-27
LK https://hdl.handle.net/20.500.14352/120027
UL https://hdl.handle.net/20.500.14352/120027
LA eng
NO Li, S.  Ferrer-Ruiz, A.  Dai, J.  Ramos-Soriano, J.  Du, X.  Zhu, M.  Zhang, W.  Wang, Y.  Herranz, M.i.  Jing, L.  Zhang, Z.  Li, H.  Xia, F.  Martín, N. A pH-independent electrochemical aptamer-based biosensor supports quantitative, real-time measurement in vivo. Chem. Sci., 2022, 13, 8813-8820.
NO Electronic supplementary information (ESI) available. See https://doi.org/10.1039/d2sc02021a
NO Spanish Ministry of Science and Innovation (MICIN)
NO National Natural Science Foundation of China
NO National Key Research and Development Program of China
NO NSFC-ISF Research Grant Program
NO Zhejiang Provincial Natural Science Foundation of China
NO Hubei Provincial Natural Science Foundation of China
DS Docta Complutense
RD 18 dic 2025