RT Journal Article
T1 Cell Therapy Based on Gingiva-Derived Mesenchymal Stem Cells Seeded in a Xenogeneic Collagen Matrix for Root Coverage of RT1 Gingival Lesions: An In Vivo Experimental Study
A1 Sanchez, Nerea
A1 Vignoletti, Fabio
A1 Sanz Martín, Ignacio
A1 Coca, Alejandro
A1 Núñez, Javier
A1 Maldonado Bautista, Estela
A1 Sanz Esporrín, Javier
A1 Hernando Pradíes, Irene
A1 Santamaría, Silvia
A1 Herrera González, David
A1 Garcia Sanz, Jose A.
A1 Sanz Alonso, Mariano
AB (1) Background: To investigate the effect of a xenogeneic collagen matrix (CMX) seeded with autologous gingiva-derived mesenchymal cells (GMSCs) when combined with a coronally advanced flap (CAF) in the treatment of localized gingival recession type 1 (RT1). (2) Methods: Dehiscence-type defects were created in seven dogs. GMSCs were isolated, transfected with a vector carrying green fluorescent protein (GFP) and expanded. Once chronified, the defects were randomly treated with (1) CAF plus the combination of CMX and GFP+ GMSCs, (2) CAF plus CMX with autologous fibroblasts, (3) CAF plus CMX and (4) CAF alone. Histological and clinical outcomes at 2- and 6-week healing periods were analyzed and compared among groups. (3) Results: Histologically, the addition of autologous cells to the CMX resulted in reduced inflammation and a variable degree of new cementum/bone formation. CMX plus GMSCs resulted in greater mean recession reduction (1.42; SD = 1.88 mm) and percentage of teeth with recession reduction of ≥2 mm (57%) when compared to the other groups, although these differences were not statistically significant. (4) Conclusions: The histometric and clinical results indicated a positive trend favouring the combination of CMX and GMSCs with the CAF when compared to the groups without cells, although these differences were not statistically significant.
PB MDPI
SN 1422-0067
YR 2022
FD 2022-03-17
LK https://hdl.handle.net/20.500.14352/73378
UL https://hdl.handle.net/20.500.14352/73378
LA eng
NO Osteology Foundation
DS Docta Complutense
RD 17 abr 2025