RT Journal Article
T1 Benchtop nuclear magnetic resonance-based metabolomicapproach for the diagnosis of bovine tuberculosis
A1 Ruiz-Cabello Osuna, Jesús
A1 Izquierdo García, José Luis
AB Even though enormous efforts and control strategies have been implemented, bovine tuberculosis (TB) remains a significant source of health and socioeconomic concern.The standard method used in TB eradication programs for in vivo detection is the tuberculin skin test. However, the specificity of the tuberculin skin test is affected by infection with non-tuberculous mycobacteria or by vaccination. Thus, some animals are not correctly diagnosed. This study aimed first to identify a plasma metabolic TB profile by high-field (HF) nuclear magnetic resonance (NMR) spectroscopy and second measure this characteristic TB metabolic profile using low-field benchtop (LF) NMR as an affordable molecular technology for TB diagnosis.Plasma samples from cattle diagnosed with TB (derivation set, n = 11), diagnosed with paratuberculosis (PTB, n = 10), PTB-vaccinated healthy control (n = 10) andhealthy PTB-unvaccinated control (n = 10) were analyzed by NMR. Unsupervised Principal Component Analysis (PCA) was used to identify metabolic differences between groups. We identified 14 metabolites significantly different between TB and control animals. The second group of TB animals was used to validate the results (validation set, n = 14). Predictive models based on metabolic fingerprint acquired by both HF and LF NMR spectroscopy successfully identified TB versus control subjects (Area under the curve of Receiver Operating Characteristic over 0.92, in both models; Confidence Interval 0.77–1).
PB Wiley & Sons
YR 2021
FD 2021
LK https://hdl.handle.net/20.500.14352/132583
UL https://hdl.handle.net/20.500.14352/132583
LA eng
NO Ruiz-Cabello, J., Sevilla, I. A., Olaizola, E., Bezos, J., Miguel-Coello, A. B., Muñoz-Mendoza, M., Beraza, M., Garrido, J. M., & Izquierdo-García, J. L. (2022). Benchtop nuclear magnetic resonance-based metabolomic approach for the diagnosis of bovine tuberculosis. Transboundary and Emerging Diseases, 69, e859–e870. https://doi.org/10.1111/tbed.14365
NO Ministerio de Ciencia e Innovación (España)
NO Ministerio de Economía, Industria y Competitividad (España)
NO Comunidad de Madrid
NO Gobierno Vasco. Dpto. Industria, Innovación, Comercio y Turismo (País Vasco)
NO European Commission
NO Fundación BBVA (España)
NO Fundación La Caixa (España)
DS Docta Complutense
RD 19 mar 2026