RT Journal Article
T1 Escherichia coli from six European countries reveals differences in profile and distribution of critical antimicrobial resistance determinants within One Health compartments, 2013 to 2020
A1 Kaspersen, Håkon P.
A1 Brouwer, Michael SM
A1 Nunez Garcia, Javier
A1 Cárdenas Rey, Ingrid
A1 AbuOun, Manal
A1 Duggett, Nicholas
A1 Ellaby, Nicholas
A1 Delgado Blas, José Francisco
A1 Hammerl, Jens A.
A1 Getino, Maria
A1 Serna Bernaldo, Carlos
A1 Naas, Thierry
A1 Veldman, Kees T.
A1 Bossers, Alex
A1 Sunde, Marianne
A1 Mo, Solveig S.
A1 Jørgensen, Silje B.
A1 Ellington, Matthew
A1 González Zorn, Bruno
A1 La Ragione, Roberto
A1 Glaser, Philippe
A1 Anjum, Muna F.
AB Background:Antimicrobial resistance (AMR) is a global threat. Monitoring using an integrated One Health approach is essential to detect changes in AMR occurrence.Aim:We aimed to detect AMR genes in pathogenic and commensal Escherichia coli collected 2013–2020 within monitoring programmes and research from food animals, food (fresh retail raw meat) and humans in six European countries, to compare vertical and horizontal transmission.Methods:We whole genome sequenced (WGS) 3,745 E. coli isolates, detected AMR genes using ResFinder and performed phylogenetic analysis to determine isolate relatedness and transmission. A BLASTn-based bioinformatic method compared draft IncI1 genomes to conserved plasmid references from Europe.Results:Resistance genes to medically important antimicrobials (MIA) such as extended-spectrum cephalosporins (ESC) were widespread but predicted resistance to MIAs authorised for human use (carbapenem, tigecycline) was detected only in two human and three cattle isolates. Phylogenetic analysis clustered E. coli according to phylogroups; commensal animal isolates showed greater diversity than those from human patients. Only 18 vertical animal-food and human-animal transmission events of E. coli clones were detected. However, IncI1 plasmids from different sources and/or countries carrying resistance to ESCs were conserved and widely distributed, although these variants were rarely detected in human pathogens.Conclusion:Using WGS we demonstrated AMR is driven vertically and horizontally. Human clinical isolates were more closely related, but their IncI1 plasmids were more diverse, while animal or food isolates were less similar with more conserved IncI1 plasmids. These differences likely arose from variations in selective pressure, influencing AMR evolution and transmission.
PB European Centre for Disease Prevention and Control
SN 1560-7917
YR 2024
FD 2024
LK https://hdl.handle.net/20.500.14352/113510
UL https://hdl.handle.net/20.500.14352/113510
LA eng
NO Kaspersen, H. P., Brouwer, M. S., Nunez-Garcia, J., Cárdenas-Rey, I., AbuOun, M., Duggett, N., Ellaby, N., Delgado-Blas, J., Hammerl, J. A., Getino, M., Serna, C., Naas, T., Veldman, K. T., Bossers, A., Sunde, M., Mo, S. S., Jørgensen, S. B., Ellington, M., Gonzalez-Zorn, B., La Ragione, R., … Anjum, M. F. (2024). Escherichia coli from six European countries reveals differences in profile and distribution of critical antimicrobial resistance determinants within One Health compartments, 2013 to 2020. Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin, 29(47), 2400295. https://doi.org/10.2807/1560-7917.ES.2024.29.47.2400295
NO Contribución de autores:HK performed the phylogenetic analysis and the transmission cluster detection. MB assembled all raw data and performed all analysis for ResFinder to detect AMR genes. JNG performed plasmid analysis, including constructing a novel tool. MFA conceived the project; MFA, JNG, MB, and HK wrote the initial manuscript, with MFA providing oversight and direction. HK, MB, JNG, ICR, MA, ND, NE, JDS, JAH, MG, CS, TN, KTV, AB, MS, SSM, SBJ, ME, BGZ, RLR, PG and MFA contributed to the overall project design, editing, and reviewing of the manuscript.
NO European Commission
NO Dutch Ministry for Agriculture, Nature and Food Quality (Países Bajos)
NO Bundesinstitut für Risikobewertung (Alemania)
NO Veterinary Medicines Directorate (Reino Unido)
NO University of Surrey (Reino Unido)
NO Higher Education Funding Council (Reino Unido)
NO Norwegian Veterinary Institute (Noruega)
DS Docta Complutense
RD 18 dic 2025