RT Journal Article
T1 Combination of bioanalytical approaches and quantitative proteomics for the elucidation of the toxicity mechanisms associated to TiO2 nanoparticles exposure in human keratinocytes
A1 Montalvo Quirós, Sandra
A1 Luque García, José Luis
AB Titanium dioxide nanoparticles (TiO2-NPs) are being used in several consumer products. The high refractive index of nano-scaled titanium dioxide particles allows them to protect from UV radiation, and so, they can be found as one of the main components of cosmetics and suncreens. Many studies have reported the potential toxicological effects associated to TiO2-NPs such as ROS generation, DNA damage, apoptosis and cell cycle arrest, among others. The continuous and systematic use of TiO2-NPs in cosmetic products requires a full comprehension of the risks involving their sustained contact with the human skin. Thus, it is important to evaluate not only the hazardous effects but to elucidate the biomolecular mechanisms involved in such effects. Based on this premises, we have evaluated the potential toxicity of TiO2-NPs using a human epithelial cell culture (HaCaT cells) as in-vitro model, together with different bioanalytical approaches and mass spectrometry-based quantitative proteomics, to gain a deeper insight into the molecular mechanisms of toxicity associated to TiO2-NPs exposure.
PB Elsevier
SN 0278-6915
YR 2019
FD 2019-03-27
LK https://hdl.handle.net/20.500.14352/105821
UL https://hdl.handle.net/20.500.14352/105821
LA eng
NO Montalvo-Quiros, S.; Luque-Garcia, J. L. Combination of bioanalytical approaches and quantitative proteomics for the elucidation of the toxicity mechanisms associated to TiO2 nanoparticles exposure in human keratinocytes. Food and Chemical Toxicology 2019, 127, 197-205 DOI:10.1016/j.fct.2019.03.036.
DS Docta Complutense
RD 7 oct 2024