RT Journal Article
T1 Low and high dietary folic acid levels perturb postnatal cerebellar morphology in growing rats
A1 Partearroyo Cediel, Teresa
A1 Pérez Miguelsanz, Juliana
A1 Peña Melián, Ángel
A1 Maestro De Las Casas, María Del Carmen
A1 Úbeda Martín, Natalia
A1 Valera Moreiras, Gregorio
AB The brain is particularly sensitive to folate metabolic disturbances, because methyl groups are critical for brain functions. This study aimed to investigate the effects of different dietary levels of folic acid (FA) on postnatal cerebellar morphology, including the architecture and organisation of the various layers. A total of forty male OFA rats (a Sprague–Dawley strain), 5 weeks old, were classified into the following four dietary groups: FA deficient (0 mg/kg FA); FA supplemented (8 mg/kg FA); FA supra-supplemented (40 mg/kg FA); and control (2 mg/kg FA) (all n 10 per group). Rats were fed ad libitum for 30 d. The cerebellum was quickly removed and processed for histological and immunohistochemical analysis. Slides were immunostained for glial fibrillary acidic protein (to label Bergmann glia), calbindin (to label Purkinje cells) and NeuN (to label post-mitotic neurons). Microscopic analysis revealed two types of defect: partial disappearance of fissures and/or neuronal ectopia, primarily in supra-supplemented animals (incidence of 80 %, P≤0·01), but also in deficient and supplemented groups (incidence of 40 %, P≤0·05), compared with control animals. The primary fissure was predominantly affected, sometimes accompanied by defects in the secondary fissure. Our findings show that growing rats fed an FA-modified diet, including both deficient and supplemented diets, have an increased risk of disturbances in cerebellar corticogenesis. Defects caused by these diets may have functional consequences in later life. The present study is the first to demonstrate that cerebellar morphological defects can arise from deficient, as well as high, FA levels in the diet.
PB Cambridge University Press
SN 0007-1145
YR 2016
FD 2016-06-14
LK https://hdl.handle.net/20.500.14352/23406
UL https://hdl.handle.net/20.500.14352/23406
LA eng
NO Partearroyo Cediel, T., Pérez Miguelsanz, J., Peña Melián, Á. et al. «Low and High Dietary Folic Acid Levels Perturb Postnatal Cerebellar Morphology in Growing Rats». British Journal of Nutrition, vol. 115, n.o 11, junio de 2016, pp. 1967-77. DOI.org (Crossref), https://doi.org/10.1017/S0007114516001008.
NO Ministerio de Ciencia, Innovación y Universidades (España)
DS Docta Complutense
RD 20 ago 2024