RT Journal Article
T1 A Cross-Sectional Study of Obesity Effects on the Metabolomic Profile of a Leptin-Resistant Swine Model
A1 Sanz-Fernandez, M. Victoria
A1 Torres-Rovira, Laura
A1 Pesantez-Pacheco, Jose L.
A1 Vázquez Gómez, Marta
A1 Garcia-Contreras, Consolacion
A1 Astiz, Susana
A1 González Bulnes, Antonio
AB Identifying metabolite signatures associated with obesity and related diseases might represent a valuable preventive and therapeutic tool to predict subjects at risk, establish an accurate prognosis, and monitor treatment success. The current cross-sectional study is aimed to evaluate the metabolite profile of diet-induced obesity in a porcine model of leptin resistance. Six Iberian female pigs prone to develop obesity (OB) were ad libitum fed a fat-enriched diet (HFD) for 82 days. Five lean Iberian sows (CON) in a maintenance diet served as controls. At the end of the dietary treatments, all animals were sacrificed, and plasma, liver, and muscle samples were immediately collected for nuclear magnetic resonance analysis. In plasma, signals corresponding to betaine, glycerophosphocholine/phosphocholine, glycine, and glutamate were decreased; and the valine signal was increased in OB sows compared to controls. Similarly, the betaine signal was decreased in the liver. No differences were detected in muscle. The observed metabolite changes suggest alterations in branched chain amino-acid metabolism and the methionine-homocysteine cycle, which have been previously associated with obesity-related diseases and type 2 diabetes in human observational studies. The current study supports the utilization of the leptin resistant Iberian pig for further interventional research in the field.
PB MDPI
SN 2218-1989
YR 2020
FD 2020-03-05
LK https://hdl.handle.net/20.500.14352/8044
UL https://hdl.handle.net/20.500.14352/8044
LA eng
DS Docta Complutense
RD 7 abr 2025