RT Journal Article
T1 16S-23S rRNA Internal Transcribed Spacer Region (ITS) Sequencing: A Potential Molecular Diagnostic Tool for Differentiating Lactococcus garvieae and Lactococcus petauri
A1 Stoppani, Nadia
A1 Colussi, Silvia
A1 Pastorino, Paolo
A1 Prearo, Marino
A1 Sciuto, Simona
A1 Altinok, Ilhan
A1 Çağrı Öztürk, Rafet
A1 Ture, Mustafa
A1 Vela Alonso, Ana Isabel
A1 Blanco Gutiérrez, María Del Mar
A1 Kotzamanidis, Charalampos
A1 Bitchava, Konstantina
A1 Malousi, Andigoni
A1 Fariano, Lucio
A1 Volpatti, Donatella
A1 Acutis, Pier Luigi
A1 Fernández-Garayzábal Fernández, José Francisco
AB Lactococcus garvieae is the etiological agent of lactococcosis, a clinically and economically significant infectious disease affecting farmed rainbow trout. L. garvieae had been considered the only cause of lactococcosis for a long time; however, L. petauri, another species of the genus Lactococcus, has lately been linked to the same disease. The genomes and biochemical profiles of L. petauri and L. garvieae have a high degree of similarity. Traditional diagnostic tests currently available cannot distinguish between these two species. The aim of this study was to use the transcribed spacer (ITS) region between 16S rRNA and 23S rRNA as a potential useful molecular target to differentiate L. garvieae from L. petauri, saving time and money compared to genomics methods currently used as diagnostic tools for accurate discrimination between these two species. The ITS region of 82 strains was amplified and sequenced. The amplified fragments varied in size from 500 to 550 bp. Based on the sequence, seven SNPs were identified that separate L. garvieae from L. petauri. The 16S-23S rRNA ITS region has enough resolution to distinguish between closely related L. garvieae and L. petauri and it can be used as a diagnostic marker to quickly identify the pathogens in a lactococcosis outbreak.
PB MDPI
YR 2023
FD 2023-05-17
LK https://hdl.handle.net/20.500.14352/104968
UL https://hdl.handle.net/20.500.14352/104968
LA eng
NO Stoppani N, Colussi S, Pastorino P, Prearo M, Sciuto S, Altinok I, et al. 16S-23S rRNA Internal Transcribed Spacer Region (ITS) Sequencing: A Potential Molecular Diagnostic Tool for Differentiating Lactococcus garvieae and Lactococcus petauri. Microorganisms 2023;11:1320. https://doi.org/10.3390/microorganisms11051320.
NO 2023 Descuento MDPI
NO SUPERTROUT
DS Docta Complutense
RD 8 jul 2025