RT Journal Article
T1 Leishmania infantum: Antiproliferative effect of recombinant plant cystatins on promastigotes and intracellular amastigotes estimated by direct counting and real-time PCR
A1 Martínez Orts, Mónica
A1 Rubio-Somoza, I.
A1 Díaz, I.
A1 Mendez, S.
A1 Alunda Rodríguez, José María
A1 Ordóñez Gutiérrez, Lara
AB Two recombinant barley cystatins, HvCPI5 and HvCPI6, have been tested in vitro against promastigotes and intracellular amastigotes of Leishmania infantum in the J774 monocytic cell line. Toxicity of cystatins for J774 cells was also determined. In addition, a comparison between direct counts of intracellular amastigotes and quantitation of burden by Q-PCR was carried out. Low concentrations (2 lM) from both cystatins were unable to inhibit promastigote replication. HvCPI5 was toxic for mammalian cells; 0.1 lM reduced by more than 50% the cell viability. On the contrary, HvCPI6 did not exhibit any toxicity for J774 cells up to 6 lM and inhibited the intracellular amastigote multiplication. Dose–response analysis showed that 4.8 lM HvCPI6 reduced by >90% the intracellular parasite load and had an approximate IC50 value of 1.5 lM. Comparable results were obtained by direct counting of intracellular amastigotes and Q-PCR. Results point towards the direct inhibition of amastigote multiplication by HvCPI6 and the interest of this recombinant cystatin in the chemotherapy of leishmaniasis.
PB Elsevier
SN 0014-4894
YR 2009
FD 2009-12
LK https://hdl.handle.net/20.500.14352/95607
UL https://hdl.handle.net/20.500.14352/95607
LA eng
NO Ordóñez-Gutiérrez L, Martínez M, Rubio-Somoza I, Díaz I, Mendez S, Alunda JM. Leishmania infantum: antiproliferative effect of recombinant plant cystatins on promastigotes and intracellular amastigotes estimated by direct counting and real-time PCR. Exp Parasitol. 2009 Dec;123(4):341-6. doi: 10.1016/j.exppara.2009.08.015. Epub 2009 Aug 31. PMID: 19723520.
DS Docta Complutense
RD 12 abr 2025