RT Journal Article
T1 Nitric Oxide Down-regulates Caveolin-3 Levels through the Interaction with Myogenin, Its Transcription Factor
A1 Martínez-Moreno, Mónica
A1 Martínez Ruiz, Antonio
A1 Álvarez-Barrientos, Alberto
A1 Gavilanes Franco, Francisco
A1 Lamas, Santiago
A1 Rodríguez Crespo, José Ignacio
AB Certain patients suffering from chronic diseases such as AIDS or cancer experience a constant cellular secretion of tumor necrosis factor   and other pro-inflammatory cytokines that results in a continuous release of nitric oxide ( NO) to the bloodstream. One immediate consequence of the  deleterious action of  NO is weight loss and the progressive destruction of muscular mass in a process known as cachexia. We have previouslyreported that caveolin-3, a specific marker of muscle cells, becomes down-regulated by the action of NO on muscular myotubes. We describe herein that the changes observed in caveolin-3 levels are due to the alteration of the DNA binding activity of the muscular transcription factor myogenin. In the presence of  NO, the binding of transcription factors from cell nuclear extracts of muscular tissues to the E boxes present in the caveolin-3 promoter become substantially reduced.When we purified recombinant myogenin and treated it with  NO donors, we could detect its S-nitrosylation by three independent methods, suggesting that very likely one of the cysteine residues of the molecule is being modified. Given the role of myogenin as a regulatory protein that determines the level of multiple muscle genes expressed during late myogenesis, our results might represent a novel mode of regulation of muscle development under conditions of nitric oxide-mediated toxicity.
SN 0021-9258
YR 2007
FD 2007-08
LK https://hdl.handle.net/20.500.14352/102742
UL https://hdl.handle.net/20.500.14352/102742
LA eng
DS Docta Complutense
RD 10 abr 2025