RT Journal Article T1 Utilization of lactose and presence of the phospho-β-galactosidase (lacG) gene in Lactococcus garvieae isolates from different sources A1 Aguado Urda, Mónica A1 Cutuli Simón, M Teresa A1 Blanco Gutiérrez, María Del Mar A1 Aspiroz, Carmen A1 Tejedor, José L A1 Gibello Prieto, Alicia A1 Fernández-Garayzábal Fernández, José Francisco AB This study evaluates the utilization of lactose (Lac) and the presence of the phospho-β-galactosidase (lacG) gene as markers for distinguishing between fish (Lac-/lacG-) and dairy isolates (Lac+/lacG+) of Lactococcus garvieae, using a panel of L. garvieae isolates from different sources. None of the fish isolates produced acid from lactose (Lac-), however Lac-/lacG- isolates were observed in pigs, cows, birds and humans. Most of the dairy isolates (77.8%) were Lac+/lacG+, but some dairy isolates did not produce acid from this sugar. Data in the present study show that the ability to metabolize lactose and the presence of the lacG gene are heterogeneously scattered among L. garvieae isolates of different sources. Therefore, the use of these criteria as markers to differentiate between L. garvieae isolates of dairy and fish origin should be considered with caution. PB Spanish Society for Microbiology SN 1618-1905 YR 2010 FD 2010-12 LK https://hdl.handle.net/20.500.14352/45183 UL https://hdl.handle.net/20.500.14352/45183 LA eng NO Ministerio de Ciencia e Innovación (MICINN) DS Docta Complutense RD 6 may 2024