RT Journal Article
T1 Butyrate-Dependent Conformational Switch Promotes Nuclear Translocation of the Mustard Allergen Sin a 1 in Human Gut Epithelial Cells.
A1 García Gordo, Rubén
A1 Parrón Ballesteros, Jorge
A1 Olmo López, Nieves
A1 Villalba Díaz, María Teresa
A1 Batanero Cremades, Eva
A1 Turnay Abad, Francisco Javier
AB Sin a 1, the major allergen from mustard seeds, is a highly stable 2S albumin capable of reaching the intestinal epithelium in its intact form. In this study, we investigated whether butyrate, a microbiota-derived short-chain fatty acid, directly interacts with Sin a 1 and modulates its cellular behavior in Caco-2 cells. Circular dichroism analyses revealed that short-chain fatty acids induce conformational changes in Sin a 1. However, fluorescence spectroscopy showed that only butyrate modified the tertiary structure of the light chain, exposing a putative nuclear localization signal and thereby promoting the importin-dependent nuclear translocation of the allergen. In the absence of butyrate, Sin a 1 remained predominantly in the cytoplasm. In addition, butyrate altered NFKB1 transcriptional responses in Sin a 1-treated cells. Our findings reveal an effect of butyrate on the intracellular trafficking of allergens that had not previously been described, suggesting a novel mechanism by which microbiota-derived metabolites may influence food allergenicity.
PB ACS
YR 2026
FD 2026-06-24
LK https://hdl.handle.net/20.500.14352/137879
UL https://hdl.handle.net/20.500.14352/137879
LA eng
NO Butyrate-Dependent Conformational Switch Promotes Nuclear Translocation of the Mustard Allergen Sin a 1 in Human Gut Epithelial Cells. Rubén G. Gordo, Jorge Parrón-Ballesteros, Nieves Olmo, Mayte Villalba, Eva Batanero, and Javier Turnay Journal of Agricultural and Food Chemistry 2026 74 (24), 19053-19065 DOI: 10.1021/acs.jafc.6c01248
NO Funding: This work was supported by grant PID2020-116692RBI00 from the Spanish Ministry of Science, Innovation and Universities (MICIU/AEI/10.13039/501100011033), and cofounded by the European Regional Development Fund.Rubeń G. Gordo and Jorge Parron-Ballesteros ́ acknowledge predoctoral UCM-Santander grants (CT15/23 and CT58/21, respectively).
NO European Regional Development Fund
NO Ministerio de Ciencia, Innovación y Universidades (España)
DS Docta Complutense
RD 20 ago 2026