RT Journal Article
T1 Identification of new allergens in macadamia nut and cross-reactivity with other tree nuts in a Spanish cohort
A1 Gutiérrez-Díaz, Gloria
A1 Betancor, Diana
A1 Parrón Ballesteros, Jorge
A1 Gordo, Rubén G.
A1 Castromil-Benito, Estela S.
A1 Haroum, Eleisa
A1 Vázquez de la Torre, María
A1 Turnay Abad, Francisco Javier
A1 Villalba Díaz, María Teresa
A1 Cuesta-Herranz, Javier
A1 Pastor Vargas, Carlos
AB The consumption of macadamia nuts has increased due to their cardioprotective and antioxidant properties. However, this rise is consistent with an increase in the cases of macadamia nut allergy, leading to severe reactions. Although two macadamia integrifolia allergens (Mac i 1 and Mac i 2) have been identified in Australian and Japanese patients, the allergenic sensitization patterns in Western European populations, particularly in Spain, remain unclear. For this purpose, seven patients with macadamia nut allergy were recruited in Spain. Macadamia nut protein extracts were prepared and, together with hazelnut and walnut extracts, were used in Western blot and inhibition assays. IgE-reactive proteins were identified using MALDI-TOF/TOF mass spectrometry (MS). Immunoblotting assays revealed various IgE-binding proteins in macadamia nut extracts. Mass spectrometry identified three new allergens: an oleosin, a pectin acetylesterase, and an aspartyl protease. Cross-reactivity studies showed that hazelnut extract but not walnut extract inhibited macadamia nut oleosin-specific IgE binding. This suggests that oleosin could be used as marker for macadamia–hazelnut cross-reactivity. The results show an allergenic profile in the Spanish cohort different from that previously detected in Australian and Japanese populations. The distinct sensitization profiles observed highlight the potential influence of dietary habits and environmental factors exposure on allergenicity.
PB MDPI
YR 2024
FD 2024-03-26
LK https://hdl.handle.net/20.500.14352/105601
UL https://hdl.handle.net/20.500.14352/105601
LA eng
NO Gutiérrez-Díaz, G.; Betancor, D.; Parrón-Ballesteros, J.; Gordo, R.G.; Castromil-Benito, E.S.; Haroun, E.; Vázquez de la Torre, M.; Turnay, J.; Villalba, M.; Cuesta-Herranz, J.; et al. Identification of New Allergens in Macadamia Nut and Cross-Reactivity with Other Tree Nuts in a Spanish Cohort. Nutrients 2024, 16, 947. https://doi.org/10.3390/nu16070947
NO Ministerio de Ciencia e Innovación (España)
DS Docta Complutense
RD 6 oct 2024