RT Journal Article
T1 Toxoplasma CRISPR/Cas9 constructs are functional for gene disruption in Neospora caninum
A1 Arranz Solís, David
A1 Regidor-Cerrillo, Javier
A1 Lourido, Sebastian
A1 Ortega Mora, Luis Miguel
A1 Saeij, Jeroen
AB Herein we describe, to our knowledge for the first time the use of the clustered regularly interspaced short palindromic repeats/CRISPR-associated gene 9 (CRISPR/Cas9) system for genome editing of Neospora caninum, an apicomplexan parasite considered one of the main causes of abortion in cattle worldwide. By using plasmids containing the CRISPR/Cas9 components adapted to the closely related parasite Toxoplasma gondii, we successfully knocked out a green fluorescent protein (GFP) in an Nc-1 GFP-expressing strain, and efficiently disrupted the NcGRA7 gene in the Nc-Spain7 isolate by insertion of a pyrimethamine resistance cassette. The successful use of this technology in N. caninum lays the foundation for an efficient, targeted gene modification tool in this parasite.
PB Elsevier
SN 0020-7519
YR 2018
FD 2018
LK https://hdl.handle.net/20.500.14352/96373
UL https://hdl.handle.net/20.500.14352/96373
LA eng
NO Arranz-Solís, D., Regidor-Cerrillo, J., Lourido, S., Ortega-Mora, L. M., & Saeij, J. P. J. (2018). Toxoplasma CRISPR/Cas9 constructs are functional for gene disruption in Neospora caninum. International journal for parasitology, 48(8), 597–600. https://doi.org/10.1016/j.ijpara.2018.03.002
NO Ministerio de Economía y Competitividad (España)
DS Docta Complutense
RD 10 abr 2025