RT Journal Article T1 Importance of Lid Hygiene Before Ocular Surgery: Qualitative and Quantitative Analysis of Eyelid and Conjunctiva Microbiota A1 Peral Cerdá, Assumpta A1 Alonso Fernández, José A1 García García, Concepción A1 Niño Rueda, Cristina A1 Calvo del Bosque, Patricia AB Objective: To evaluate the efficacy of a cleansing eyelid wipe in reducing the microbiota present on the ocular surface before cataract surgery.Methods: A single-center, prospective, single-blind phase IV study was conducted at the University Complutense of Madrid. Forty-five adult patients who were scheduled for ocular surgery after treatment with commercially available eyelid wipes were consecutively enrolled. The study lasted 5 days and the patients were examined at day 0 (D0), day 3 (D3), and day 5 (D5). They received instructions to apply the eyelid wipe only to the eye subject to surgery, using the other eye as a control with no treatment. Lid and conjunctival swabs were taken on each day and microbes identified. Ocular surface microbiota was estimated by measuring the area of the agar plate occupied by the grown colonies with respect to the total available area.Results: Measurements at D3 and D5 showed a percent reduction of 58% and 63%, respectively, in the microbial load on the eyelid in the treated eyes (P=0.0011). There was also a reduction, although nonsignificant, in the microbiota of the conjunctiva of 72% and 69% on D3 and D5, respectively.Conclusions: The degree of microbiota reduction was comparable with that obtained after topical application of antibiotics in other studies. The results suggest the use of these eyelid wipes as a complementary prophylactic method before any ocular surgery. PB Contact Lens Association of Ophthalmologists, Inc. SN 1542-2321 YR 2016 FD 2016-11 LK https://hdl.handle.net/20.500.14352/17627 UL https://hdl.handle.net/20.500.14352/17627 LA eng NO Accepted October 05, 2015.Presented at the Association for Research in Vision and Ophthalmology (ARVO) 2014 Annual Meeting, May 4–8, 2014, Orlando, FL.Open Access editor Lippincott Willians & Wilkins NO Thea Laboratories (Barcelona, Spain) DS Docta Complutense RD 8 may 2024