RT Journal Article
T1 Ghrelin modulates gene and protein expression of digestive enzymes in the intestine and hepatopancreas of goldfish (Carassius auratus) via the GHS-R1a: Possible roles of PLC/PKC and AC/PKA intracellular signaling pathways
A1 Blanco Imperiali, Ayelén M.
A1 Bertucci, Juan I.
A1 Sánchez Bretaño, Aída
A1 Delgado Saavedra, María Jesús
A1 Valenciano González, Ana Isabel
A1 Unniappan, Suraj
AB Ghrelin, a multifunctional gut-brain hormone, is involved in the regulation of gastric functions in mammals. This study aimed to determine whether ghrelin modulates digestive enzymes in goldfish (Carassius auratus). Immunofluorescence microscopy found colocalization of ghrelin, GHS-R1a and the digestive enzymes sucrase-isomaltase, aminopeptidase A, trypsin and lipoprotein lipase in intestinal and hepatopancreatic cells. In vitro ghrelin treatment in intestinal and hepatopancreas explant culture led to a concentration- and time-dependent modulation (mainly stimulatory) of most of the digestive enzymes tested. The ghrelin-induced upregulations of digestive enzyme expression were all abolished by preincubation with the GHS-R1a ghrelin receptor antagonist [D-Lys3]-GHRP-6, and most of them by the phospholipase C inhibitor U73122 or the protein kinase A inhibitor H89. This indicates that ghrelin effects on digestive enzymes are mediated by GHS-R1a, partly by triggering the PLC/PKC and AC/PKA intracellular signaling pathways. These data suggest a role for ghrelin on digestive processes in fish.
PB Elsevier
SN 0303-7207
YR 2017
FD 2017-02-15
LK https://hdl.handle.net/20.500.14352/18022
UL https://hdl.handle.net/20.500.14352/18022
LA eng
NO Ministerio de Economía y Competitividad (MINECO)
NO Ministerio de Educación y Ciencia (MEC)
NO Natural Sciences and Engineering Research Council (NSERC) (Canada)
NO Canada Foundation for Innovation
NO Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), (Argentina)
DS Docta Complutense
RD 25 ago 2024