RT Journal Article T1 Identification and Molecular Characterization of Four New Blastocystis Subtypes Designated ST35-ST38 A1 Maloney, Jenny G. A1 Molokin, Aleksey A1 Seguí, Raimundo A1 Maravilla, Pablo A1 Martínez Hernández, Fernando A1 Villalobos, Guiehdani A1 Tsaousis, Anastasios D. A1 Gentekaki, Eleni A1 Muñoz-Antolí, Carla A1 Klisiowicz, Debora R. A1 Oishi, Camila Y. A1 Toledo, Rafael A1 Esteban, J. Guillermo A1 Köster, Pamela C. A1 de Lucio, Aida A1 Dashti, Alejandro A1 Bailo, Begoña A1 Calero Bernal, Rafael A1 González Barrio, David A1 Carmena, David A1 Santín, Mónica AB Three recent studies of Blastocystis epidemiology in mammalian hosts identified four novel sequences that appeared to share B. lapemi as the most similar sequence. However, full-length ssu rRNA gene sequences were not available to confirm the validity of these new subtypes. In the present study, Nanopore MinION sequencing was used to obtain full-length reference sequences for each of the new subtypes. Additionally, phylogenetic analyses and pairwise distance comparisons were performed to confirm the validity of each of these new subtypes. We propose that the novel sequences described in this study should be assigned the subtype designations ST35-ST38. The full-length reference sequences of ST35-ST38 will assist in accurate sequence descriptions in future studies of Blastocystis epidemiology and subtype diversity. PB MPDI SN 2076-2607 YR 2022 FD 2022-12-23 LK https://hdl.handle.net/20.500.14352/73199 UL https://hdl.handle.net/20.500.14352/73199 LA eng NO USDA-ARS/ NO Instituto de Salud Carlos III/ FEDER/RICET NO Instituto de Salud Carlos III DS Docta Complutense RD 12 abr 2025