RT Journal Article
T1 Connections between the internal and the external capsules and the globus pallidus in the sheep: A dichromate stain X‐ray microtomographic study
A1 Murillo González, Jorge Alfonso
A1 Notario, Belen
A1 Maldonado Bautista, Estela
A1 Martínez Sanz, Elena
A1 Barrio Asensio, María Del Carmen
A1 Herrera Lara, Manuel Eugenio
AB Sheep are recognized as useful species for translational neurodegeneration research, in particular for the study of Huntington disease. There is a lack of information regarding the detailed anatomy and connections of the basal ganglia of sheep, in normal myeloarchitectonics and in tract-tracing studies. In this work, the organization of the corticostriatal projections at the level of the putamen and globus pallidus (GP) are explored. For the first time, the myeloarchitectonic pattern of connections between the internal (IC) and the external (EC) capsules with the GP have been investigated in the sheep. Formaldehyde-fixed blocks of the striatum were treated with a metallic stain containing potassium dichromate and visualized using micro-CT (µ-CT). The trivalent chromium (Cr3+), attached to myelin phospholipids, imparts a differential contrast to the grey and white matter compartments, which allows the visualization of myelinated fascicles in µ-CT images. The fascicles were classified according to their topographical location in dorsal supreme fascicles (X, Y, apex) arising from the IC and EC; pre-commissurally, basal fascicles connecting the ventral part of the EC with the lateral zone of the ventral pallidum (VP) and, post-commissurally, superior (Z1 ), middle (Z2 ) and lower (Z3 ) fascicles, connecting at different levels the EC with the GP. The results suggest that the presumptive cortical efferent and afferent fibres to the pallidum could be organized according to a dorsal to ventrolateral topography in the sheep, similar to that seen in other mammals. The proposed methodology has the potential to delineate the myeloarchitectonic patterns of nervous systems and tracts.
PB WILEY
SN 0340-2096
SN 1439-0264
YR 2020
FD 2020-08-13
LK https://hdl.handle.net/20.500.14352/102502
UL https://hdl.handle.net/20.500.14352/102502
LA eng
NO Murillo-González JA, Notario B, Maldonado E, Martinez-Sanz E, Barrio MC, Herrera M. Connections between the internal and the external capsules and the globus pallidus in the sheep: A dichromate stain X-ray microtomographic study. Anat Histol Embryol. 2021 Jan;50(1):84-92.
DS Docta Complutense
RD 19 jul 2024