RT Journal Article
T1 Anti-Trichomonas gallinae activity of essential oils and main compounds from Lamiaceae and Asteraceae plants
A1 Bailén, María
A1 Díaz Castellanos, Irene
A1 Azami Conesa, Iris
A1 Alonso Fernández, Sara
A1 Martínez Díaz, Rafael A.
A1 Navarro Rocha, Juliana
A1 Gómez Muñoz, María Teresa
A1 González Coloma, Azucena
AB Trichomonas gallinae is a flagellated protozoan that parasitizes the upper digestive tract of various bird species and causes avian trichomonosis. The emergence of resistant strains to the standard treatment, based on nitroimidazoles, increases the need to find alternative therapies. In this study, 36 essential oils (EOs) from Lamiaceae and Asteraceae plant families were tested against T. gallinae trophozoites using the 3-(4,5-dimethylthiazol-2-yl-)-2,5-dipheniltetrazolium bromide (MTT) reduction assay. Among them, EOs from distinct species of Lamiaceae, including the genera Lavandula, Salvia, Thymus, Origanum, and Satureja were the ones reporting better anti-trichomonal activity, and were selected for further analysis, including chemical composition and in vitro assays. The chemical composition of the selected EOs was determined by gas chromatography followed by mass spectrometry and 19 pure compounds were tested against the protozoa, according to their higher abundance in the active EOs. Pure compounds which displayed the highest activity against T. gallinae trophozoites, ordered by highest to lowest activity, were α and β-thujones, camphene, β-pinene, linalyl acetate, thymol, 4-terpineol, γ-terpinene, α-pinene, p-cymene, D-fenchone and β-caryophyllene. A dose dependent effect was observed in most of the EOs and pure compounds tested. The toxicity test conducted in eukaryotic cell cultures with the anti-trichomonal active pure compounds showed that β-caryophyllene, camphene, α-pinene, and β-pinene were slightly toxic for Vero cells, and the selectivity index was calculated. Based on the anti-trichomonal activity and the absence of cytotoxicity results, natural products from Lamiaceae plants could be useful as alternative therapy against avian trichomonosis, mainly those containing linalyl acetate, thymol, 4-terpinenol, γ-terpinene, p-cymene and D-fenchone.
PB Frontiers Media
SN 2297-1769
YR 2022
FD 2022-09-09
LK https://hdl.handle.net/20.500.14352/72621
UL https://hdl.handle.net/20.500.14352/72621
LA eng
NO Ministerio de Ciencia e Innovación (MICINN); 10.13039/501100011033)
NO Universidad Complutense de Madrid/Banco de Santander
DS Docta Complutense
RD 12 may 2025