RT Journal Article
T1 KATP channel opening accelerates and stabilizes rotors in a swine heart model of ventricular fibrillation
A1 Quintanilla, Jorge G.
A1 Moreno, Javier
A1 Archondo, Tamara
A1 Pérez Castellano, Nicasio
A1 Usandizaga, Elena
A1 García Torrent, María Jesús
A1 Molina Morúa, Roberto
A1 González, Pablo
A1 Rodríguez Bobada, Cruz
A1 Macaya Miguel, Carlos
A1 Pérez Villacastín Domínguez, Julián
AB AimsThe mechanisms underlying ventricular fibrillation (VF) are still disputed. Recent studies have highlighted the role of KATP-channels. We hypothesized that, under certain conditions, VF can be driven by stable and epicardially detectable rotors in large hearts. To test our hypothesis, we used a swine model of accelerated VF by opening KATP-channels with cromakalim.Methods and resultsOptical mapping, spectral analysis, and phase singularity tracking were performed in eight perfused swine hearts during VF. Pseudo-bipolar electrograms were computed. KATP-channel opening almost doubled the maximum dominant frequency (14.3 ± 2.2 vs. 26.5 ± 2.8 Hz, P < 0.001) and increased the maximum regularity index (0.82 ± 0.05 vs. 0.94 ± 0.04, P < 0.001), the density of rotors (2.0 ± 1.4 vs. 16.0 ± 7.0 rotors/cm2×s, P < 0.001), and their maximum lifespans (medians: 368 vs. ≥3410 ms, P < 0.001). Persistent rotors (≥1 movie = 3410 ms) were found in all hearts after cromakalim (mostly coinciding with the fastest and highest organized areas), but they were not epicardially visible at baseline VF. A ‘beat phenomenon’ ruled by inter-domain frequency gradients was observed in all hearts after cromakalim. Acceleration of VF did not reveal any significant regional preponderance. Complex fractionated electrograms were not found in areas near persistent rotors.ConclusionUpon KATP-channel opening, VF consisted of rapid and highly organized domains mainly due to stationary rotors, surrounded by poorly organized areas. A ‘beat phenomenon’ due to the quasi-periodic onset of drifting rotors was observed. These findings demonstrate the feasibility of a VF driven by stable rotors in hearts whose size is similar to the human heart. Our model also showed that complex fractionation does not seem to localize stationary rotors.
PB Oxford University Press
SN 0008-6363
YR 2023
FD 2023-08-01
LK https://hdl.handle.net/20.500.14352/100399
UL https://hdl.handle.net/20.500.14352/100399
LA eng
NO Quintanilla JG, Moreno J, Archondo T, Chin A, Pérez-Castellano N, Usandizaga E, García-Torrent MJ, Molina-Morúa R, González P, Rodríguez-Bobada C, Macaya C, Pérez-Villacastín J. KATP channel opening accelerates and stabilizes rotors in a swine heart model of ventricular fibrillation. Cardiovasc Res. 2013 Aug 1;99(3):576-85. doi: 10.1093/cvr/cvt093
NO El artículo habla del estudio realizado que investiga los mecanismos que causan la fibrilación ventricular (FV), un ritmo cardíaco peligroso. Se centra en el papel de los canales KATP y su relación con la formación de rotores estables durante la FV. Se realizó un experimento en ocho corazones de cerdo, donde se observó que la apertura de los canales KATP aumentaba la frecuencia cardíaca y la organización de los rotores durante la FV. Estos rotores estables no eran visibles externamente en la fase inicial de la FV. Además, se observó un fenómeno de batido, que sugiere la presencia de rotores en movimiento. Los resultados indican que la FV puede ser impulsada por rotores estables en corazones grandes, con implicaciones importantes para comprender y tratar este trastorno cardíaco.
NO Fundación Mutua Madrileña
NO Instituto de Salud Carlos III
NO Unión Europea
DS Docta Complutense
RD 9 abr 2025