RT Journal Article
T1 Manufactured silver and gold nanoparticles-induced apoptosis by caspase-pathway in human cell lines
A1 Alicia Ávalos, 
A1 Morales Gómez, Paloma
A1 Haza Duaso, Ana Isabel
AB Metallic nanoparticles have emerged as an important class of nanomaterials for a wide range of industrial and medical applications. Because of the intensive commercial applications, risk assessment of these nanoparticles is of great importance. In the present study, the human hepatoma and leukemia cells were used to characterize the apoptotic effects of silver nanoparticles (4.7 and 42 nm) and gold nanoparticles (30 nm). Apoptotic cells were identified by chromatin condensation and flow cytometry analysis, using Annexin V/PI, TUNEL and caspase activation assays. Flow cytometry analysis showed that the three metallic nanoparticles induced apoptotic cell death in a concentration and time dependent-manner. Moreover, the three nanoparticles induced activation of caspase-3 and -7 in hepatoma and leukemia cells. Apoptotic effects were stronger after exposure of both cell lines with 4.7 nm silver nanoparticles than those obtained with 42 nm silver and 30 nm gold nanoparticles. In conclusion, silver (4.7 and 42 nm) and gold (30 nm) nanoparticles induced apoptosis in hepatoma and leukemia cells via the caspase dependent pathway. The smaller silver nanoparticles (4.7 nm) had a greater ability to induce apoptosis in both cell lines.
PB Taylor & Francis
SN 0277-2248
SN 1029-0486
YR 2019
FD 2019-06-14
LK https://hdl.handle.net/20.500.14352/93433
UL https://hdl.handle.net/20.500.14352/93433
LA eng
NO Alicia Ávalos, Paloma Morales & Ana Isabel Haza (2018) Manufactured silver and gold nanoparticles-induced apoptosis by caspase-pathway in human cell lines, Toxicological & Environmental Chemistry, 100:5-7, 629-643, DOI: 10.1080/02772248.2018.1545127
NO Ministerio de Educación, Cultura y Deporte (AGL2010-16561)
DS Docta Complutense
RD 28 jul 2024