%0 Journal Article
%A De la Rocha-Muñoz, Andrés
%A Benito Villalvilla, Cristina
%A Olivares, David
%A Sirvent Bernal, Sofía
%A García-Brenes, Miguel Ángel
%A Angelina Querencias, Alba
%A Martín De La Cruz, Leticia
%A Cuesta, Javier
%A Tassinari, Paolo
%A Jaumont, Xavier
%A Taxonera, Carlos
%A Palomares Gracia, Óscar
%T The role of IgE in Crohn's disease by impairing the capacity of plasmacytoid dendritic cells to generate FOXP3+ Tregs
%D 2025
%U https://hdl.handle.net/20.500.14352/120388
%X Background: A causal relationship between Crohn's disease (CD) and asthma is reported, but the underlying mechanisms are not fully understood. We sought to investigate the role of IgE and IgE-mediated pathways in the pathophysiology of CD.Methods: 20 CD patients, 10 allergic patients without inflammatory bowel disease, and 10 healthy donors (HD) were included in the study. Total serum IgE was quantified by ELISA. Circulating IgE+ and FcεRIα+ immune cells, as well as specific CD4+ T cell populations, were determined by flow cytometry. Gene set enrichment signatures from available single-cell (sc)RNAseq datasets of the intestine from CD patients were analyzed. Purified plasmacytoid dendritic cells (pDCs) from CD patients were cocultured with naïve CD4+ T cells to assess Tregs generation.Results: CD patients, similar to allergic non-CD patients, displayed significantly higher numbers of circulating IgE+ or FcεRIα+ immune cells than HD. The percentage of blood IgE+ or FcεRIα+ pDCs was significantly higher in CD than HD and similar to allergic non-CD patients. CD patients showed significantly higher numbers of effector memory CD4+ T cells and lower numbers of FOXP3+ Tregs than HD. scRNAseq data from CD patients confirmed that Tregs imbalance and overactivation of IgE-mediated pathways take place also in gut tissues of children and adults, suggesting IgE could interfere in the pDC-Tregs axis. In vitro functional experiments demonstrated that IgE-crosslinking on pDCs from CD patients impairs Treg generation, which was restored by the anti-IgE mAb omalizumab.Conclusions: IgE might play an unprecedented role in CD by impairing the capacity of pDCs to generate Tregs, which could represent a novel mechanism contributing to CD to be exploited for alternative therapeutic interventions.
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