RT Journal Article
T1 Fertilizing capacity of vitrified stallion sperm assessed utilizing heterologous IVF after different semen warming procedures
A1 Consuegra González, Cesar
A1 Crespo Castejón, Francisco
A1 Dorado Martín, Jesús
A1 María Díaz Jiménez, 
A1 Sánchez Calabuig, María Jesús
A1 Beltrán Breña, Paula
A1 Pérez Cerezales, Serafín
A1 Rizos, Dimitri
A1 Hidalgo Prieto, 
AB The aim of this study was to evaluate the fertilizing capacity of frozen or vitrified stallion sperm after assessing different warming procedures. In Experiment 1, different warming procedures were compared after sperm vitrification: immersion in extender at 43 ◦C (C), or in a water bath at 37 ◦C/30 s (W37), 43 ◦C/10 s (W43) or 60 ◦C/5 s (W60). With the W60 treatment, there were greater values (P < 0.05) for VCL (83.93 ± 3.6 μm/s) and ALH (3.00 ± 0.2 μm) than freezing and with the C group, and greater values (P < 0.001) for PM (35.33 ± 2.5 %) than with the W43 treatment. In Experiment 2, the fertilizing capacity of vitrified and frozen sperm was assessed utilizing heterologous IVF procedures, using cattle oocytes. Vitrification resulted in greater values (P < 0.05) than freezing for the number of bound sperm (1.36 ± 0.3 and 0.69 ± 0.2, respectively). There were no differences between frozen or vitrified sperm in pronuclear formation (26 hours post-insemination - hpi; 14.08 ± 4.2 % and 22.78 ± 4.8 %, respectively) or cleavage rate (32.77 ± 4.3 % and 39.66 ± 4.6 %, respectively). In conclusion, vitrified stallion sperm warmed in a water bath at 60 ºC had the capacity to penetrate cattle oocytes, leading to pronuclear formation and hybrid embryo cleavage after heterologous IVF.
PB Elsevier
SN 0378-4320
YR 2020
FD 2020-12
LK https://hdl.handle.net/20.500.14352/97208
UL https://hdl.handle.net/20.500.14352/97208
LA eng
NO Consuegra C, Crespo F, Dorado J, Diaz-Jimenez M, Pereira B, Sánchez-Calabuig MJ, Beltrán-Breña P, Pérez-Cerezales S, Rizos D, Hidalgo M. Fertilizing capacity of vitrified stallion sperm assessed utilizing heterologous IVF after different semen warming procedures. Anim Reprod Sci. 2020 Dec;223:106627
NO FPU Spanish fellowship (16/05745) from MECD (“Ministerio de Educaci´on, Cultura y Deporte”).
DS Docta Complutense
RD 27 sept 2024