RT Journal Article
T1 The kinase ATR controls meiotic crossover distribution at the genome scale in Arabidopsis
A1 Zhu, Longfei
A1 Dluzewska, Julia
A1 Fernández Jiménez, Nadia
A1 Ranjan, Rajeev
A1 Pelé, Alexandre
A1 Dziegielewski, Wojciech
A1 Szymanska-Lejman, Maja
A1 Hus, Karolina
A1 Górna, Julia
A1 Pradillo Orellana, Mónica
A1 Ziolkowski, Piotr A.
AB Meiotic crossover, i.e. the reciprocal exchange of chromosome fragments during meiosis, is a key driver of genetic diversity. Crossover is initiated by the formation of programmed DNA double-strand breaks (DSBs). While the role of ATAXIA-TELANGIECTASIA AND RAD3-RELATED (ATR) kinase in DNA damage signaling is well-known, its impact on crossover formation remains understudied. Here, using measurements of recombination at chromosomal intervals and genome-wide crossover mapping, we showed that ATR inactivation in Arabidopsis (Arabidopsis thaliana) leads to dramatic crossover redistribution, with an increase in crossover frequency in chromosome arms and a decrease in pericentromeres. These global changes in crossover placement were not caused by alterations in DSB numbers, which we demonstrated by analyzing phosphorylated H2A.X foci in zygonema. Using the seed-typing technique, we found that hotspot usage remains mainly unchanged in atr mutants compared with wild-type individuals. Moreover, atr showed no change in the number of crossovers caused by two independent pathways, which implies no effect on crossover pathway choice. Analyses of genetic interaction indicate that while the effects of atr are independent of MMS AND UV SENSITIVE81 (MUS81), ZIPPER1 (ZYP1), FANCONI ANEMIA COMPLEMENTATION GROUP M (FANCM), and D2 (FANCD2), the underlying mechanism may be similar between ATR and FANCD2. This study extends our understanding of ATR's role in meiosis, uncovering functions in regulating crossover distribution.
PB Oxford University Press
SN 1040-4651
YR 2024
FD 2024
LK https://hdl.handle.net/20.500.14352/111140
UL https://hdl.handle.net/20.500.14352/111140
LA eng
NO Longfei Zhu, Julia Dluzewska, Nadia Fernández-Jiménez, Rajeev Ranjan, Alexandre Pelé, Wojciech Dziegielewski, Maja Szymanska-Lejman, Karolina Hus, Julia Górna, Mónica Pradillo, Piotr A Ziolkowski, The kinase ATR controls meiotic crossover distribution at the genome scale in Arabidopsis, The Plant Cell, 2024;, koae292, https://doi.org/10.1093/plcell/koae292
NO This work was supported by the National Science Center Poland grants 2016/22/E/NZ2/00455 and 2020/39/I/NZ2/02464 to P.A.Z. The computations were carried out in the Poznan Supercomputing and Networking Center (grants 312 andpl0074-02). W.D. and M.Sz.L. are the holders of Foundation for Polish Science stipends (START 2023).
NO National Science Center Poland
NO Poznan Supercomputing and Networking Center
NO Ministerio de Ciencia e Innovación (España)
NO European Commission
NO Foundation for Polish Science
DS Docta Complutense
RD 6 abr 2025