RT Journal Article
T1 Localization of alkali-labile sites in donkey (Equus asinus) and stallion (Equus caballus) spermatozoa
A1 Gosálvez Berenguer, Jaime
A1 Gálvez, MJ
A1 Acha Valls, Daniel
A1 Gosálvez Berenguer, Jaime
A1 Crespo Castejón, Francisco
AB The presence of constitutive alkali-labile sites (ALS) has been investigated using a protocol of DNA breakage detection-fluorescence in situ hybridization and comet assay in spermatozoa of donkey (Equus asinus) and stallion (Equus caballus). These results were compared with those obtained using a similar experimental approach using somatic cells. The relative abundance of ALS was of the order of four times more in spermatozoa than in somatic cells. Alkali-labile sites showed a tendency to cluster localized at the equatorial-distal regions of the sperm. The amount of hybridized signal in the ALS in the sperm of donkey (Equus asinus) was 1.3 times greater than in stallion (Equus caballus), and the length of the comet tail obtained in donkey sperm was 1.6 times longer than that observed in stallion (P < 0.05); however, these differences were not appreciated in somatic cells. In conclusion, ALS localization in sperm is not a randomized event and a different pattern of ALS distribution occurs for each species. These results suggest that ALS represents a species-specific issue related to chromatin organization in sperm and somatic cells in mammalian species, and they might diverge even with very short phylogenetic distances.
PB Elsevier
SN 0093-691X
YR 2014
FD 2014-01
LK https://hdl.handle.net/20.500.14352/97379
UL https://hdl.handle.net/20.500.14352/97379
LA eng
NO 6)	Cortés-Gutiérrez EI, Dávila-Rodríguez MI, López-Fernández C, Fernández JL, Crespo F, Gosálvez J. Localization of alkali-labile sites in donkey (Equus asinus) and stallion (Equus caballus) spermatozoa. Theriogenology. 2014 Jan 15;81(2):321-5.
DS Docta Complutense
RD 27 sept 2024