RT Journal Article
T1 PLGA cationic nanoparticles, obtained from nano-emulsion templating, as potential DNA vaccines
A1 Soler Besumbes, Eduard
A1 Fornaguera, Cristina
A1 Monge, Marta
A1 García-Celma, María José
A1 Carrión Herrero, Francisco Javier
A1 Solans, Conxita
A1 Dols-Perez, Aurora
AB Polymeric nanoparticles offer advantageous characteristics as gene-delivery vectors such as biocompatibility and biodegradability. With this aim, a smart and innovative strategy was followed here: Cationic PLGA nano-emulsions, prepared by a low energy method, were used as templates to obtain cationic nanoparticles (NPs) able to easily complex with nucleic acids (i.e. plasmid DNA) by electrostatic interactions. The strategy employed to produce stable positively-charged nanoparticles was the use of non-ionic/cationic surfactant mixtures to stabilize template nano-emulsions. This methodology allowed obtaining nanoparticles with reproducible nanometric sizes and positive zeta potential values, appropriate to successfully complex with nucleic acids, resulting in nanometric spherical polyplexes. Nanoparticles, plasmids and polyplexes proved to be biocompatible at the optimal concentration. Therefore, we can conclude that we have designed a novel strategy to efficiently obtain cationic polymeric nanoparticles that can be a promising approach to act as novel non-viral gene-delivery vectors, useful for many applications in gene therapy, such as gene vaccines.
PB Elsevier
SN 0014-3057
YR 2019
FD 2019
LK https://hdl.handle.net/20.500.14352/93695
UL https://hdl.handle.net/20.500.14352/93695
LA eng
NO Besumbes, E. S., Fornaguera, C., Monge, M., García-Celma, M. J., Carrión, J., Solans, C., & Dols-Pérez, A. (2019). PLGA cationic nanoparticles, obtained from nano-emulsion templating, as potential DNA vaccines. European Polymer Journal, 120, 109229. https://doi.org/10.1016/j.eurpolymj.2019.109229
NO Ministerio de Economía y Competitividad (España)
NO Generalitat de Catalunya
NO Instituto de Salud Carlos III
NO European Commission
DS Docta Complutense
RD 6 abr 2025