RT Journal Article
T1 Correlations of telomere length, telomeraseactivity, and telomeric-repeat binding factor 1expression in colorectal carcinoma. Prognostic indications
A1 Garcia‐Aranda, Cristina
A1 Diaz‐Lopez, Antonio
A1 Pilar Iniesta, 
A1 Juan Chocano, María Del Carmen De
A1 Sánchez Pernaute, Andrés
A1 Torres García, Antonio José
A1 Díaz-Rubio García, Eduardo
A1 Balibrea Cantero, José Luis
A1 Benito De Las Heras, Manuel R.
A1 Iniesta Serrano, María Pilar
AB BACKGROUNDTelomere maintenance has been proposed as an essential step for tumor cell immortalization. The objectives of the current study were to investigate the mechanisms implicated in telomere length in colorectal carcinoma (CRC) and to evaluate the prognostic impact of telomere status.METHODSNinety‐one colorectal carcinoma samples that were obtained from patients who underwent surgery were analyzed to investigate the factors related to telomere function. The authors studied telomerase activity, terminal restriction fragment (TRF) length, and telomeric‐repeat binding factor (TRF1) expression and analyzed the prognostic implications of those factors.RESULTSMost tumors (81.3%) displayed telomerase activity. Overall, telomeres in CRC specimens were significantly shorter compared with telomeres in normal, adjacent specimens (P = 0.02). Moreover, tumors that demonstrated shortened telomeres displayed higher TRF1 levels than tumors without telomere shortening. In relation to patient prognosis, a significantly poor clinical course was observed in the group of patients who had tumors with longer telomeres (P = 0.02), and this finding emerged as an independent prognostic factor in a Cox proportional hazards model (P = 0.04; relative risk, 6.48). Among patients with tumors classified as telomerase‐positive, telomere length ratios (the ratio of tumor tissues to normal tissues) ≤ 0.66 or TRF1 over‐expression conferred a favorable outcome (P = 0.03 and P = 0.05, respectively).CONCLUSIONSThe majority of CRC specimens in the current study displayed telomerase reactivation. However, only those tumors that displayed telomere elongation conferred a poor prognosis. Conversely, colorectal tumors that over‐expressed TRF1 demonstrated telomere shortening, and patients with those tumors had a better clinical course. Cancer 2006. © 2005 American Cancer Society.
PB WILEY
SN 0008-543X
YR 2005
FD 2005-12-30
LK https://hdl.handle.net/20.500.14352/116206
UL https://hdl.handle.net/20.500.14352/116206
LA eng
NO Garcia-Aranda C, de Juan C, Diaz-Lopez A, Sanchez-Pernaute A, Torres AJ, Diaz-Rubio E, Balibrea JL, Benito M, Iniesta P. Correlations of telomere length, telomerase activity, and telomeric-repeat binding factor 1 expression in colorectal carcinoma. Cancer. 2006 Feb 1;106(3):541-51. doi: 10.1002/cncr.21625. PMID: 16388518.
DS Docta Complutense
RD 28 abr 2025