%0 Journal Article
%A Al‐Hamed, Faez Saleh
%A Abu‐Nada, Lina
%A Rodan, Rania
%A Sarrigiannidis, Stylianos
%A Ramirez‐Garcialuna, José Luis
%A Moussa, Hanan
%A Elkashty, Osama
%A Gao, Qiman
%A Basiri, Tayebeh 
%A Baca González, Laura
%A Torres García Denche, Jesús
%A Rancán, Lisa
%A Tran, Simon D.
%A Lordkipanidzé, Marie 
%A Kaartinen, Mari 
%A Badran, Zahi 
%A Tamimi, Faleh 
%T Differences in platelet‐rich plasma composition influence bone healing
%D 2021
%@ 0303-6979
%@ 1600-051X
%U https://hdl.handle.net/20.500.14352/93312
%X Aim: Platelet-rich plasma (PRP) is an autologous blood-derived material that has been used to enhance bone regeneration. Clinical studies, however, reported inconsistent outcomes. This study aimed to assess the effect of changes in leucocyte and PRP(L-PRP) composition on bone defect healing.Materials and Methods: L-PRPs were prepared using different centrifugation methods and their regenerative potential was assessed in an in-vivo rat model. Bilateral critical-size tibial bone defects were created and filled with single-spin L-PRP, double-spin L-PRP, or filtered L-PRP. Empty defects and defects treated with collagen scaffolds served as controls. Rats were euthanized after 2 weeks, and their tibias were collected and analysed using micro-CT and histology.Results: Double-spin L-PRP contained higher concentrations of platelets than singlespin L-PRP and filtered L-PRP. Filtration of single-spin L-PRP resulted in lower concentrations of minerals and metabolites. In vivo, double-spin L-PRP improved bone healing by significantly reducing the size of bone defects (1.08 ± 0.2 mm3) compared to single-spin L-PRP (1.42 ± 0.27 mm3) or filtered L-PRP (1.38 ± 0.28 mm3). There were fewer mast cells, lymphocytes, and macrophages in defects treated with double-spin L-PRP than in those treated with single-spin or filtered L-PRP.Conclusion: The preparation method of L-PRP affects their composition and potential to regenerate bone.
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